Adjuvants for porcine reproductive and respiratory syndrome virus vaccines.

This review deals with present and past efforts in utilization of vaccine adjuvants for porcine reproductive and respiratory syndrome virus (PRRSV) vaccines. PRRSV vaccines elicit delayed and weak cell-mediated immune (CMI) and antibody responses after vaccination. Several kinds of vaccine adjuvants have been utilized to accelerate and magnify immune responses to PRRSV vaccines. These adjuvants include cytokines, chemical reagents, and bacterial products. Of 11 vaccine adjuvants tested, five (i.e. interleukin-2 (IL-2), IL-12, interferon alpha (IFNalpha), polyinosinic and polycytidylic acid, and cytidine-phosphate-guanosine oligodeoxynucleotides (CpG ODN)) significantly enhance CMI response to PRRSV vaccines. The response is characterized by proliferation, cytotoxicity, and IFNgamma secretion of peripheral blood mononuclear cells or T cells in response to recall PRRSV antigens in vitro. Two (i.e. CpG ODN and cholera toxin) significantly enhance PRRSV-specific antibody response after vaccination. Two (i.e. IL-2 and CpG ODN) significantly enhance protective efficacy of PRRSV vaccines in challenge models. Improvement of immune responses to PRRSV vaccines should focus in future studies on assessing more vaccine adjuvants for their efficiency in enhancing both CMI and antibody responses and on identifying PRRSV components and strategies that down-modulate pig immune responses in order to devise vaccine adjuvants that can regulate such strategies of the virus.