PURPOSE: Interstitial cystitis is a chronic pelvic pain syndrome of which the origin and mechanisms involved remain unclear. In this study Ca(2+) transients in the bladder wall of domestic cats diagnosed with naturally occurring feline interstitial cystitis were examined. MATERIALS AND METHODS: Cross-sections of full-thickness bladder strips from normal cats and cats with feline interstitial cystitis were examined by optically mapping Ca(2+) transients and recording tension. Responses of Ca(2+) activity and detrusor contractions to pharmacological interventions were compared. In addition, pharmacological responses were compared in mucosa denuded preparations. RESULTS: Optical mapping showed that feline interstitial cystitis bladders had significantly more spontaneous Ca(2+) transients in the mucosal layer than control bladders. Optical mapping also demonstrated that feline interstitial cystitis bladders were hypersensitive to a low dose (50 nM) of the muscarinic receptor agonist arecaidine when the mucosal layer was intact. This hypersensitivity was markedly decreased in mucosa denuded bladder strips. CONCLUSIONS: In feline interstitial cystitis cat bladders there is increased Ca(2+) activity and sensitivity of muscarinic receptors in the mucosal layer, which can enhance smooth muscle spontaneous contractions.
PURPOSE:Interstitial cystitis is a chronic pelvic pain syndrome of which the origin and mechanisms involved remain unclear. In this study Ca(2+) transients in the bladder wall of domestic cats diagnosed with naturally occurring feline interstitial cystitis were examined. MATERIALS AND METHODS: Cross-sections of full-thickness bladder strips from normal cats and cats with feline interstitial cystitis were examined by optically mapping Ca(2+) transients and recording tension. Responses of Ca(2+) activity and detrusor contractions to pharmacological interventions were compared. In addition, pharmacological responses were compared in mucosa denuded preparations. RESULTS: Optical mapping showed that feline interstitial cystitis bladders had significantly more spontaneous Ca(2+) transients in the mucosal layer than control bladders. Optical mapping also demonstrated that feline interstitial cystitis bladders were hypersensitive to a low dose (50 nM) of the muscarinic receptor agonist arecaidine when the mucosal layer was intact. This hypersensitivity was markedly decreased in mucosa denuded bladder strips. CONCLUSIONS: In feline interstitial cystitis cat bladders there is increased Ca(2+) activity and sensitivity of muscarinic receptors in the mucosal layer, which can enhance smooth muscle spontaneous contractions.
Authors: C A Buffington; D J Chew; M S Kendall; P V Scrivani; S B Thompson; J L Blaisdell; B E Woodworth Journal: J Am Vet Med Assoc Date: 1997-01-01 Impact factor: 1.936
Authors: L A Birder; H Z Ruan; B Chopra; Z Xiang; S Barrick; C A Buffington; J R Roppolo; A P D W Ford; W C de Groat; G Burnstock Journal: Am J Physiol Renal Physiol Date: 2004-07-13
Authors: Lori A Birder; Michele L Nealen; Susanna Kiss; William C de Groat; Michael J Caterina; Edward Wang; Gerard Apodaca; Anthony J Kanai Journal: J Neurosci Date: 2002-09-15 Impact factor: 6.167