Literature DB >> 19156792

Proteomic analysis of plasma membrane vesicles.

Brigitte Bauer1, Max Davidson, Owe Orwar.   

Abstract

A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flowcell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived.

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Year:  2009        PMID： 19156792     DOI： 10.1002/anie.200803898

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  16 in total

1.  Temperature-dependent phase behavior and protein partitioning in giant plasma membrane vesicles.

Authors:  S A Johnson; B M Stinson; M S Go; L M Carmona; J I Reminick; X Fang; T Baumgart
Journal:  Biochim Biophys Acta       Date:  2010-03-15

2.  Single Molecule Imaging Deciphers the Relation between Mobility and Signaling of a Prototypical G Protein-coupled Receptor in Living Cells.

Authors:  Luc Veya; Joachim Piguet; Horst Vogel
Journal:  J Biol Chem       Date:  2015-09-11       Impact factor: 5.157

3.  Pulsed-laser creation and characterization of giant plasma membrane vesicles from cells.

Authors:  Christopher V Kelly; Mary-Margaret T Kober; Päivö Kinnunen; David A Reis; Bradford G Orr; Mark M Banaszak Holl
Journal:  J Biol Phys       Date:  2009-06-20       Impact factor: 1.365

Review 4.  Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.

Authors:  Ida Louise Jørgensen; Gerdi Christine Kemmer; Thomas Günther Pomorski
Journal:  Eur Biophys J       Date:  2016-07-20       Impact factor: 1.733

5.  HAMLET interacts with lipid membranes and perturbs their structure and integrity.

Authors:  Ann-Kristin Mossberg; Maja Puchades; Øyvind Halskau; Anne Baumann; Ingela Lanekoff; Yinxia Chao; Aurora Martinez; Catharina Svanborg; Roger Karlsson
Journal:  PLoS One       Date:  2010-02-23       Impact factor: 3.240

6.  Single-virus fusion experiments reveal proton influx into vaccinia virions and hemifusion lag times.

Authors:  Florian I Schmidt; Phillip Kuhn; Tom Robinson; Jason Mercer; Petra S Dittrich
Journal:  Biophys J       Date:  2013-07-16       Impact factor: 4.033

7.  Membrane fluctuations and acidosis regulate cooperative binding of 'marker of self' protein CD47 with the macrophage checkpoint receptor SIRPα.

Authors:  Jan Steinkühler; Bartosz Różycki; Cory Alvey; Reinhard Lipowsky; Thomas R Weikl; Rumiana Dimova; Dennis E Discher
Journal:  J Cell Sci       Date:  2018-07-16       Impact factor: 5.285

8.  A membrane-delimited N-myristoylated CRMP2 peptide aptamer inhibits CaV2.2 trafficking and reverses inflammatory and postoperative pain behaviors.

Authors:  Liberty François-Moutal; Yue Wang; Aubin Moutal; Karissa E Cottier; Ohannes K Melemedjian; Xiaofang Yang; Yuying Wang; Weina Ju; Tally M Largent-Milnes; May Khanna; Todd W Vanderah; Rajesh Khanna
Journal:  Pain       Date:  2015-07       Impact factor: 7.926

9.  Formation of raft-like assemblies within clusters of influenza hemagglutinin observed by MD simulations.

Authors:  Daniel L Parton; Alex Tek; Marc Baaden; Mark S P Sansom
Journal:  PLoS Comput Biol       Date:  2013-04-11       Impact factor: 4.779

10.  Downscaling the analysis of complex transmembrane signaling cascades to closed attoliter volumes.

Authors:  Luigino Grasso; Romain Wyss; Joachim Piguet; Michael Werner; Ghérici Hassaïne; Ruud Hovius; Horst Vogel
Journal:  PLoS One       Date:  2013-08-05       Impact factor: 3.240
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