Literature DB >> 19153449

Crystallization and preliminary X-ray diffraction analysis of GCIP/HHM transcriptional regulator.

Azusa Seto1, Hiroaki Ikushima, Toshiyasu Suzuki, Yusuke Sato, Shuya Fukai, Keiko Yuki, Keiji Miyazawa, Kohei Miyazono, Ryuichiro Ishitani, Osamu Nureki.   

Abstract

GCIP/HHM is a human nuclear protein that is implicated in regulation of cell proliferation. Its primary structure contains helix-loop-helix and leucine-zipper motifs but lacks a DNA-binding basic region. Native and selenomethionine-derivatized (SeMet) crystals of full-length GCIP/HHM were obtained using the hanging-drop vapour-diffusion method. The crystals were greatly improved by adding tris(2-carboxyethyl)phosphine as a reducing reagent and diffracted to 3.5 A resolution. Preliminary phase calculations using the data set obtained from the SeMet crystal suggested that the crystal belonged to space group P3(2)21 and contained one molecule per asymmetric unit. Structure determination by the multiple-wavelength anomalous dispersion method using the SeMet crystals is in progress.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 19153449      PMCID： PMC2628855          DOI： 10.1107/S1744309108038219

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

Review 1.  Role of Id family proteins in growth control.

Authors:  Yoshifumi Yokota; Seiichi Mori
Journal:  J Cell Physiol       Date:  2002-01       Impact factor: 6.384

Review 2.  Id proteins in cell cycle control and cellular senescence.

Authors:  Z Zebedee; E Hara
Journal:  Oncogene       Date:  2001-12-20       Impact factor: 9.867

3.  Methods used in the structure determination of bovine mitochondrial F1 ATPase.

Authors:  J P Abrahams; A G Leslie
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1996-01-01

4.  Maid (GCIP) is involved in cell cycle control of hepatocytes.

Authors:  Eva Sonnenberg-Riethmacher; Torsten Wüstefeld; Michaela Miehe; Christian Trautwein; Dieter Riethmacher
Journal:  Hepatology       Date:  2007-02       Impact factor: 17.425

5.  Id proteins Id1 and Id2 selectively inhibit DNA binding by one class of helix-loop-helix proteins.

Authors:  X H Sun; N G Copeland; N A Jenkins; D Baltimore
Journal:  Mol Cell Biol       Date:  1991-11       Impact factor: 4.272

6.  The protein Id: a negative regulator of helix-loop-helix DNA binding proteins.

Authors:  R Benezra; R L Davis; D Lockshon; D L Turner; H Weintraub
Journal:  Cell       Date:  1990-04-06       Impact factor: 41.582

7.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

8.  Human homologue of maid is a useful marker protein in hepatocarcinogenesis.

Authors:  Taro Takami; Shuji Terai; Yuichiro Yokoyama; Haruko Tanimoto; Kunihiko Tajima; Koichi Uchida; Takahiro Yamasaki; Isao Sakaida; Hiroshi Nishina; Snorri S Thorgeirsson; Kiwamu Okita
Journal:  Gastroenterology       Date:  2005-05       Impact factor: 22.682

9.  Sequence-specific DNA binding by the c-Myc protein.

Authors:  T K Blackwell; L Kretzner; E M Blackwood; R N Eisenman; H Weintraub
Journal:  Science       Date:  1990-11-23       Impact factor: 47.728

10.  Human homologue of maid: A dominant inhibitory helix-loop-helix protein associated with liver-specific gene expression.

Authors:  S Terai; H Aoki; K Ashida; S S Thorgeirsson
Journal:  Hepatology       Date:  2000-08       Impact factor: 17.425
View more
  4 in total

1.  Crystallization and preliminary X-ray diffraction studies of the GhKCH2 motor domain: alteration of pH significantly improved the quality of the crystals.

Authors:  Xinghua Qin; Ziwei Chen; Tao Xu; Ping Li; Guoqin Liu
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-06-28

2.  Structure of a dominant-negative helix-loop-helix transcriptional regulator suggests mechanisms of autoinhibition.

Authors:  Ryohei Ishii; Kazunobu Isogaya; Azusa Seto; Daizo Koinuma; Yuji Watanabe; Fumio Arisaka; So-ichi Yaguchi; Hiroaki Ikushima; Naoshi Dohmae; Kohei Miyazono; Keiji Miyazawa; Ryuichiro Ishitani; Osamu Nureki
Journal:  EMBO J       Date:  2012-03-27       Impact factor: 11.598

3.  Involvement of DNA Damage Response via the Ccndbp1-Atm-Chk2 Pathway in Mice with Dextran-Sodium-Sulfate-Induced Colitis.

Authors:  Ryoko Horigome; Kenya Kamimura; Yusuke Niwa; Kohei Ogawa; Ken-Ichi Mizuno; Koichi Fujisawa; Naoki Yamamoto; Taro Takami; Tomoyuki Sugano; Akira Sakamaki; Hiroteru Kamimura; Masaaki Takamura; Shuji Terai
Journal:  J Clin Med       Date:  2022-06-25       Impact factor: 4.964

4.  Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway.

Authors:  Yusuke Niwa; Kenya Kamimura; Kohei Ogawa; Chiyumi Oda; Yuto Tanaka; Ryoko Horigome; Masato Ohtsuka; Hiromi Miura; Koichi Fujisawa; Naoki Yamamoto; Taro Takami; Shujiro Okuda; Masayoshi Ko; Takashi Owaki; Atsushi Kimura; Osamu Shibata; Shinichi Morita; Norihiro Sakai; Hiroyuki Abe; Takeshi Yokoo; Akira Sakamaki; Hiroteru Kamimura; Shuji Terai
Journal:  J Clin Med       Date:  2022-02-06       Impact factor: 4.241
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.