Literature DB >> 19152049

Large-scale gene disruption using the UAU1 cassette.

Clarissa J Nobile1, Aaron P Mitchell.   

Abstract

Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.

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Year:  2009        PMID： 19152049      PMCID： PMC2786983          DOI： 10.1007/978-1-60327-151-6_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

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3.  A single-transformation gene function test in diploid Candida albicans.

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4.  Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.

Authors:  R B Wilson; D Davis; A P Mitchell
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5.  Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis.

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  5 in total
  33 in total

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2.  Genetic control of Candida albicans biofilm development.

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Journal:  Methods Mol Biol       Date:  2011

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Review 5.  Functional profiling of human fungal pathogen genomes.

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Journal:  Cold Spring Harb Perspect Med       Date:  2014-11-06       Impact factor: 6.915

6.  Candida albicans SH3-domain proteins involved in hyphal growth, cytokinesis, and vacuolar morphology.

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7.  Interface of Candida albicans biofilm matrix-associated drug resistance and cell wall integrity regulation.

Authors:  Jeniel E Nett; Hiram Sanchez; Michael T Cain; Kelly M Ross; David R Andes
Journal:  Eukaryot Cell       Date:  2011-06-10

8.  Haplotyping a Non-meiotic Diploid Fungal Pathogen Using Induced Aneuploidies and SNP/CGH Microarray Analysis.

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Journal:  Methods Mol Biol       Date:  2017

9.  Role of filamentation in Galleria mellonella killing by Candida albicans.

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Journal:  Microbes Infect       Date:  2010-03-09       Impact factor: 2.700

10.  A model system for mitochondrial biogenesis reveals evolutionary rewiring of protein import and membrane assembly pathways.

Authors:  Victoria L Hewitt; Eva Heinz; Miguel Shingu-Vazquez; Yue Qu; Branka Jelicic; Tricia L Lo; Traude H Beilharz; Geoff Dumsday; Kipros Gabriel; Ana Traven; Trevor Lithgow
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