Literature DB >> 19150352

Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite.

Pete Gagnon1, Chia-Wei Cheung, Paul J Yazaki.   

Abstract

This study introduces the application of calcium-derivatized hydroxyapatite for purification of Fab. Fab binds to native hydroxyapatite but fails to bind to the calcium derivatized form. IgG, Fc, and most other protein contaminants bind to the calcium form. This supports Fab purification by a simple flow-through method that achieves greater than 95% purity from papain digests and mammalian cell culture supernatants. Alternatively, Fab can be concentrated on native hydroxyapatite then eluted selectively by conversion to the calcium-derivatized form.

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Year:  2009        PMID： 19150352      PMCID： PMC3171993          DOI： 10.1016/j.jim.2008.11.021

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

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9.  The interaction of proteins with hydroxyapatite. I. Role of protein charge and structure.

Authors:  M J Gorbunoff
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365
  9 in total
  4 in total

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  4 in total

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