Literature DB >> 19148603

Propagation and culture of renal fibroblasts.

Lauren Grimwood1, Rosemary Masterson.   

Abstract

In this chapter we describe a reliable and reproducible method for the selective propagation and culture of renal fibroblasts derived from explantation of renal cortical tissue in vitro. The chapter outlines how primary renal interstitial fibroblasts are derived from explants grown in medium supplemented with foetal calf serum. The subculture of confluent cells and their ultimate characterisation as fibroblasts through immunohistochemical and immunocytochemical techniques are described in detail.

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Year:  2009        PMID： 19148603     DOI： 10.1007/978-1-59745-352-3_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Experimental inhibition of porcupine-mediated Wnt O-acylation attenuates kidney fibrosis.

Authors:  Babita Madan; Mehul B Patel; Jiandong Zhang; Ralph M Bunte; Nathan P Rudemiller; Robert Griffiths; David M Virshup; Steven D Crowley
Journal:  Kidney Int       Date:  2016-03-25       Impact factor: 10.612

2.  Inactivation of MAP3K7 in FOXD1-expressing cells results in loss of mesangial PDGFRΒ and juvenile kidney scarring.

Authors:  Michele J Karolak; Justin A Guay; Leif Oxburgh
Journal:  Am J Physiol Renal Physiol       Date:  2018-04-18

3.  Cannabinoid receptor 1 is a major mediator of renal fibrosis.

Authors:  Lola Lecru; Christophe Desterke; Stanislas Grassin-Delyle; Christos Chatziantoniou; Sophie Vandermeersch; Aurore Devocelle; Amelia Vernochet; Ninoslav Ivanovski; Catherine Ledent; Sophie Ferlicot; Meriem Dalia; Myriam Saïd; Séverine Beaudreuil; Bernard Charpentier; Aimé Vazquez; Julien Giron-Michel; Bruno Azzarone; Antoine Durrbach; Hélène François
Journal:  Kidney Int       Date:  2015-03-11       Impact factor: 10.612

4.  MicroRNA-22 is a master regulator of bone morphogenetic protein-7/6 homeostasis in the kidney.

Authors:  Jianyin Long; Shawn S Badal; Yin Wang; Benny H J Chang; Antony Rodriguez; Farhad R Danesh
Journal:  J Biol Chem       Date:  2013-10-25       Impact factor: 5.157

5.  Yap/Taz Deletion in Gli+ Cell-Derived Myofibroblasts Attenuates Fibrosis.

Authors:  Ming Liang; Michael Yu; Ruohan Xia; Ke Song; Jun Wang; Jinlong Luo; Guang Chen; Jizhong Cheng
Journal:  J Am Soc Nephrol       Date:  2017-08-02       Impact factor: 10.121

6.  Instructive Role of the Microenvironment in Preventing Renal Fibrosis.

Authors:  Kei Matsumoto; Sandhya Xavier; Jun Chen; Yujiro Kida; Mark Lipphardt; Reina Ikeda; Annie Gevertz; Mario Caviris; Antonis K Hatzopoulos; Ivo Kalajzic; James Dutton; Brian B Ratliff; Hong Zhao; Zbygniew Darzynkiewicz; Stefan Rose-John; Michael S Goligorsky
Journal:  Stem Cells Transl Med       Date:  2016-10-05       Impact factor: 6.940

7.  Epigenetic Modifications to H3K9 in Renal Tubulointerstitial Cells after Unilateral Ureteric Obstruction and TGF-β1 Stimulation.

Authors:  Timothy D Hewitson; Stephen G Holt; Sven-Jean Tan; Belinda Wigg; Chrishan S Samuel; Edward R Smith
Journal:  Front Pharmacol       Date:  2017-05-29       Impact factor: 5.810

8.  FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts.

Authors:  Edward R Smith; Sven-Jean Tan; Stephen G Holt; Tim D Hewitson
Journal:  Sci Rep       Date:  2017-06-13       Impact factor: 4.379

9.  The Anti-fibrotic Actions of Relaxin Are Mediated Through a NO-sGC-cGMP-Dependent Pathway in Renal Myofibroblasts In Vitro and Enhanced by the NO Donor, Diethylamine NONOate.

Authors:  Chao Wang; Barbara K Kemp-Harper; Martina Kocan; Sheng Yu Ang; Tim D Hewitson; Chrishan S Samuel
Journal:  Front Pharmacol       Date:  2016-03-31       Impact factor: 5.810

10.  Human Liver Stem Cell-Derived Extracellular Vesicles Prevent Aristolochic Acid-Induced Kidney Fibrosis.

Authors:  Sharad Kholia; Maria Beatriz Herrera Sanchez; Massimo Cedrino; Elli Papadimitriou; Marta Tapparo; Maria Chiara Deregibus; Maria Felice Brizzi; Ciro Tetta; Giovanni Camussi
Journal:  Front Immunol       Date:  2018-07-19       Impact factor: 7.561
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