OBJECTIVES: The precise role of gonadotropins in the carcinogenesis of epithelial ovarian cancer remains uncertain. Recently, the haplotype of two single nucleotide polymorphisms, Thr307Ala (rs6165) and Asn680Ser (rs6166), has been described as a risk factor for ovarian cancer in Chinese women. In this study we investigated the impact of this haplotype regarding the risk to develop ovarian cancer as well as possible effects upon the clinical course in a Caucasian patient sample. SUBJECTS AND METHODS: Determination of genotypes in 115 patients with primary epithelial ovarian cancer and 115 age-matched controls was performed by Pyrosequencing for Thr307Ala and by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for Asn680Ser. RESULTS: Analysis of the genotypes revealed almost complete linkage disequilibrium of both SNPs. The distribution of genotypes was not statistically significant different between ovarian cancer patients and age-matched controls. Clinical parameters such as overall survival, CA12-5 elevation at primary diagnosis, age at diagnosis, FIGO stage, grading, and platinum resistance were not statistically significantly different regarding genotypes. CONCLUSIONS: We could not confirm the FSHR Ala307-Ser680 haplotype as a risk factor for epithelial ovarian cancer in Caucasian women. Hence, the modification of tumor risk may be affected by the ethnology of the patient collective. We could not find any associations of clinical parameters or course of the disease with the different genotypes.
OBJECTIVES: The precise role of gonadotropins in the carcinogenesis of epithelial ovarian cancer remains uncertain. Recently, the haplotype of two single nucleotide polymorphisms, Thr307Ala (rs6165) and Asn680Ser (rs6166), has been described as a risk factor for ovarian cancer in Chinese women. In this study we investigated the impact of this haplotype regarding the risk to develop ovarian cancer as well as possible effects upon the clinical course in a Caucasian patient sample. SUBJECTS AND METHODS: Determination of genotypes in 115 patients with primary epithelial ovarian cancer and 115 age-matched controls was performed by Pyrosequencing for Thr307Ala and by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for Asn680Ser. RESULTS: Analysis of the genotypes revealed almost complete linkage disequilibrium of both SNPs. The distribution of genotypes was not statistically significant different between ovarian cancerpatients and age-matched controls. Clinical parameters such as overall survival, CA12-5 elevation at primary diagnosis, age at diagnosis, FIGO stage, grading, and platinum resistance were not statistically significantly different regarding genotypes. CONCLUSIONS: We could not confirm the FSHRAla307-Ser680 haplotype as a risk factor for epithelial ovarian cancer in Caucasian women. Hence, the modification of tumor risk may be affected by the ethnology of the patient collective. We could not find any associations of clinical parameters or course of the disease with the different genotypes.
Authors: Konstantinos Papadimitriou; Panteleimon Kountourakis; Anastasia E Kottorou; Anna G Antonacopoulou; Christian Rolfo; Marc Peeters; Haralabos P Kalofonos Journal: Mol Diagn Ther Date: 2016-12 Impact factor: 4.074
Authors: Alice W Lee; Jonathan P Tyrer; Jennifer A Doherty; Douglas A Stram; Jolanta Kupryjanczyk; Agnieszka Dansonka-Mieszkowska; Joanna Plisiecka-Halasa; Beata Spiewankiewicz; Emily J Myers; Georgia Chenevix-Trench; Peter A Fasching; Matthias W Beckmann; Arif B Ekici; Alexander Hein; Ignace Vergote; Els Van Nieuwenhuysen; Diether Lambrechts; Kristine G Wicklund; Ursula Eilber; Shan Wang-Gohrke; Jenny Chang-Claude; Anja Rudolph; Lara Sucheston-Campbell; Kunle Odunsi; Kirsten B Moysich; Yurii B Shvetsov; Pamela J Thompson; Marc T Goodman; Lynne R Wilkens; Thilo Dörk; Peter Hillemanns; Matthias Dürst; Ingo B Runnebaum; Natalia Bogdanova; Liisa M Pelttari; Heli Nevanlinna; Arto Leminen; Robert P Edwards; Joseph L Kelley; Philipp Harter; Ira Schwaab; Florian Heitz; Andreas du Bois; Sandra Orsulic; Jenny Lester; Christine Walsh; Beth Y Karlan; Estrid Hogdall; Susanne K Kjaer; Allan Jensen; Robert A Vierkant; Julie M Cunningham; Ellen L Goode; Brooke L Fridley; Melissa C Southey; Graham G Giles; Fiona Bruinsma; Xifeng Wu; Michelle A T Hildebrandt; Karen Lu; Dong Liang; Maria Bisogna; Douglas A Levine; Rachel Palmieri Weber; Joellen M Schildkraut; Edwin S Iversen; Andrew Berchuck; Kathryn L Terry; Daniel W Cramer; Shelley S Tworoger; Elizabeth M Poole; Sara H Olson; Irene Orlow; Elisa V Bandera; Line Bjorge; Ingvild L Tangen; Helga B Salvesen; Camilla Krakstad; Leon F A G Massuger; Lambertus A Kiemeney; Katja K H Aben; Anne M van Altena; Yukie Bean; Tanja Pejovic; Melissa Kellar; Nhu D Le; Linda S Cook; Linda E Kelemen; Angela Brooks-Wilson; Jan Lubinski; Jacek Gronwald; Cezary Cybulski; Anna Jakubowska; Nicolas Wentzensen; Louise A Brinton; Jolanta Lissowska; Hannah Yang; Lotte Nedergaard; Lene Lundvall; Claus Hogdall; Honglin Song; Ian G Campbell; Diana Eccles; Rosalind Glasspool; Nadeem Siddiqui; Karen Carty; James Paul; Iain A McNeish; Weiva Sieh; Valerie McGuire; Joseph H Rothstein; Alice S Whittemore; John R McLaughlin; Harvey A Risch; Catherine M Phelan; Hoda Anton-Culver; Argyrios Ziogas; Usha Menon; Susan J Ramus; Aleksandra Gentry-Maharaj; Patricia Harrington; Malcolm C Pike; Francesmary Modugno; Mary Anne Rossing; Roberta B Ness; Paul D P Pharoah; Daniel O Stram; Anna H Wu; Celeste Leigh Pearce Journal: Gynecol Oncol Date: 2014-12-17 Impact factor: 5.482