AIM: To evaluate the different tissues of naturally contaminated oyster for food-borne virus detection. METHODS AND RESULTS: The different tissues of 136 field oyster samples were analysed for norovirus (NV), hepatitis A virus (HAV) and rotavirus (RV) by reverse transcription (RT)-PCR and were confirmed by sequencing. These viruses were detected in 20 samples (14.71%), showing positivity for NV (1.47%), HAV (5.15%) and RV (8.82%). Furthermore, among different tissues, the highest positive rate of the food-borne viruses was found in the gills (14.71%), followed by the stomach (13.97%) and the digestive diverticula (13.24%). CONCLUSIONS: The food-borne viruses were detected in the gills, stomach, digestive diverticula and the cilia of the mantle. In addition, the results showed that the gills are one of the appropriate tissues for viral detection in oysters by nucleic acid assay. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first paper to report on the presence of food-borne viruses in the gills and the cilia of the mantle of naturally contaminated oysters. The research team hopes that the results of the study will be of help in sampling the appropriate tissues for the detection of food-borne viruses in commercial oysters.
AIM: To evaluate the different tissues of naturally contaminated oyster for food-borne virus detection. METHODS AND RESULTS: The different tissues of 136 field oyster samples were analysed for norovirus (NV), hepatitis A virus (HAV) and rotavirus (RV) by reverse transcription (RT)-PCR and were confirmed by sequencing. These viruses were detected in 20 samples (14.71%), showing positivity for NV (1.47%), HAV (5.15%) and RV (8.82%). Furthermore, among different tissues, the highest positive rate of the food-borne viruses was found in the gills (14.71%), followed by the stomach (13.97%) and the digestive diverticula (13.24%). CONCLUSIONS: The food-borne viruses were detected in the gills, stomach, digestive diverticula and the cilia of the mantle. In addition, the results showed that the gills are one of the appropriate tissues for viral detection in oysters by nucleic acid assay. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first paper to report on the presence of food-borne viruses in the gills and the cilia of the mantle of naturally contaminated oysters. The research team hopes that the results of the study will be of help in sampling the appropriate tissues for the detection of food-borne viruses in commercial oysters.
Authors: Keleigh Provost; Brooke A Dancho; Gulnihal Ozbay; Robert S Anderson; Gary P Richards; David H Kingsley Journal: Appl Environ Microbiol Date: 2011-09-23 Impact factor: 4.792
Authors: Haifa Maalouf; Maha Zakhour; Jacques Le Pendu; Jean-Claude Le Saux; Robert L Atmar; Françoise S Le Guyader Journal: Appl Environ Microbiol Date: 2010-06-18 Impact factor: 4.792
Authors: David Rodríguez-Lázaro; Nigel Cook; Franco M Ruggeri; Jane Sellwood; Abid Nasser; Maria Sao Jose Nascimento; Martin D'Agostino; Ricardo Santos; Juan Carlos Saiz; Artur Rzeżutka; Albert Bosch; Rosina Gironés; Annalaura Carducci; Michelle Muscillo; Katarina Kovač; Marta Diez-Valcarce; Apostolos Vantarakis; Carl-Henrik von Bonsdorff; Ana Maria de Roda Husman; Marta Hernández; Wim H M van der Poel Journal: FEMS Microbiol Rev Date: 2011-10-24 Impact factor: 16.408