BACKGROUND AND OBJECTIVES: It is unknown if the decreased ability to relax airways smooth muscles in asthma and other inflammatory disorders, such as acute respiratory distress syndrome (ARDS), can be influenced by low level laser therapy (LLLT) irradiation. In this context, the present work was developed in order to investigate if LLLT could reduce dysfunction in inflamed bronchi smooth muscles (BSM) in rats. STUDY DESIGN/ MATERIALS AND METHODS: A controlled ex vivo study was developed where bronchi from Wistar rat were dissected and mounted in an organ bath apparatus with or without a TNF-alpha. RESULTS: LLLT administered perpendicularly to a point in the middle of the dissected bronchi with a wavelength of 655 nm and a dose of 2.6 J/cm(2), partially decreased BSM hyperreactivity to cholinergic agonist, restored BSM relaxation to isoproterenol and reduced the TNF-alpha mRNA expression. An NF-kappaB antagonist (BMS205820) blocked the LLLT effect on dysfunction in inflamed BSM. CONCLUSION: The results obtained in this work indicate that the LLLT effect on alterations in responsiveness of airway smooth muscles observed in TNF-alpha-induced experimental acute lung inflammation seems to be dependent of NF-kappaB activation.
BACKGROUND AND OBJECTIVES: It is unknown if the decreased ability to relax airways smooth muscles in asthma and other inflammatory disorders, such as acute respiratory distress syndrome (ARDS), can be influenced by low level laser therapy (LLLT) irradiation. In this context, the present work was developed in order to investigate if LLLT could reduce dysfunction in inflamed bronchi smooth muscles (BSM) in rats. STUDY DESIGN/ MATERIALS AND METHODS: A controlled ex vivo study was developed where bronchi from Wistar rat were dissected and mounted in an organ bath apparatus with or without a TNF-alpha. RESULTS: LLLT administered perpendicularly to a point in the middle of the dissected bronchi with a wavelength of 655 nm and a dose of 2.6 J/cm(2), partially decreased BSM hyperreactivity to cholinergic agonist, restored BSM relaxation to isoproterenol and reduced the TNF-alpha mRNA expression. An NF-kappaB antagonist (BMS205820) blocked the LLLT effect on dysfunction in inflamed BSM. CONCLUSION: The results obtained in this work indicate that the LLLT effect on alterations in responsiveness of airway smooth muscles observed in TNF-alpha-induced experimental acute lung inflammation seems to be dependent of NF-kappaB activation.
Authors: Flávia Mafra de Lima; Leonardo M Moreira; A B Villaverde; Regiane Albertini; Hugo C Castro-Faria-Neto; Flávio Aimbire Journal: Lasers Med Sci Date: 2010-12-24 Impact factor: 3.161
Authors: Flávia Mafra de Lima; Jan M Bjordal; Regiane Albertini; Fábio V Santos; Flavio Aimbire Journal: Lasers Med Sci Date: 2010-09 Impact factor: 3.161
Authors: Flávia Mafra de Lima; Flávio Aimbire; Humberto Miranda; Rodolfo de Paula Vieira; Ana Paula Ligeiro de Oliveira; Regiane Albertini Journal: J Lasers Med Sci Date: 2014
Authors: Feng Lin; Steven F Josephs; Doru T Alexandrescu; Famela Ramos; Vladimir Bogin; Vincent Gammill; Constantin A Dasanu; Rosalia De Necochea-Campion; Amit N Patel; Ewa Carrier; David R Koos Journal: J Transl Med Date: 2010-02-16 Impact factor: 5.531
Authors: Kristianne Porta Santos Fernandes; Agnelo Neves Alves; Fábio Daumas Nunes; Nadhia Helena Costa Souza; José Antônio Silva; Sandra Kalil Bussadori; Raquel Agnelli Mesquita Ferrari Journal: Lasers Med Sci Date: 2012-11-23 Impact factor: 3.161