| Literature DB >> 19142888 |
Shikha Bose1, Lee-Fah Yap, Matthew Fung, Jane Starzcynski, Amyza Saleh, Susan Morgan, Christopher Dawson, Marilyn B Chukwuma, Esther Maina, Maike Buettner, Wenbin Wei, John Arrand, Paul V H Lim, Lawrence S Young, Soo Hwang Teo, Tatjana Stankovic, Ciaran B J Woodman, Paul G Murray.
Abstract
A micro-array analysis using biopsies from patients with EBV-positive undifferentiated nasopharyngeal carcinoma (NPC) and from cancer-free controls revealed down-regulation of tumour suppressor genes (TSG) not previously associated with this disease; one such gene was the ataxia telangiectasia mutated (ATM) gene. Q-PCR confirmed down-regulation of ATM mRNA and ATM protein expression in tumour cells was weak or absent in almost all cases. In NPC cell lines, however, ATM was down-regulated only in the EBV-positive line, C666.1, and in none of five EBV-negative lines. In vitro infection of EBV-negative NPC cell lines with a recombinant EBV was followed by the down-regulation of ATM mRNA and protein, and only EBV-positive cells showed a defective DNA damage response following gamma-irradiation. Our data suggest that loss of ATM function could be an important step in the pathogenesis of NPC, and may have implications for the treatment of this disease.Entities:
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Year: 2009 PMID： 19142888 DOI： 10.1002/path.2487
Source DB: PubMed Journal: J Pathol ISSN： 0022-3417 Impact factor: 7.996