Ya-Mei Yu1, Chin-Huei Lin, Hsu-Chin Chan, Hong-Der Tsai. 1. Dept. of Health Science, Chang Jung Christian University, 396 Chang Jung Rd., Sec. 1, Kway Jen, Tainan 71101, Taiwan. yuyamei@hotmail.com
Abstract
BACKGROUND: Expression of cell adhesion molecules on the endothelium and the attachment of monocytes to endothelium may play a major role in the early atherogenic process. AIM OF THE STUDY: We investigated the effects of carnosic acid on the adhesion of U937 cells to IL-1beta-treated human umbilical vein endothelial cells (HUVECs), as well as on the expression of adhesion molecules. RESULTS: Our data showed that pretreatment with 10 and 20 micromol/l carnosic acid significantly reduced the number of U937 cells adhering to IL-1beta-treated HUVECs. In addition, we found that 20 micromol/l carnosic was more effective than 10 micromol/l carnosic acid at inhibiting expression of cell adhesion molecules (ICAM-1, VCAM-1, and E-selectin), the nuclear translocation of NF-kappaB subunits p65 and p50, and the production of ROS in IL-1beta-stimulated HUVECs. CONCLUSIONS: We conclude that carnosic acid inhibits IL-1beta-induced ICAM-1, VCAM-1 and E-selectin expression in HUVECs through a mechanism that involves NFkappaB. We propose that the reduction in binding of human monocytic cell line U937 to IL-1beta-treated HUVECs is due to the anti-inflammatory properties of carnosic acid.
BACKGROUND: Expression of cell adhesion molecules on the endothelium and the attachment of monocytes to endothelium may play a major role in the early atherogenic process. AIM OF THE STUDY: We investigated the effects of carnosic acid on the adhesion of U937 cells to IL-1beta-treated human umbilical vein endothelial cells (HUVECs), as well as on the expression of adhesion molecules. RESULTS: Our data showed that pretreatment with 10 and 20 micromol/l carnosic acid significantly reduced the number of U937 cells adhering to IL-1beta-treated HUVECs. In addition, we found that 20 micromol/l carnosic was more effective than 10 micromol/l carnosic acid at inhibiting expression of cell adhesion molecules (ICAM-1, VCAM-1, and E-selectin), the nuclear translocation of NF-kappaB subunits p65 and p50, and the production of ROS in IL-1beta-stimulated HUVECs. CONCLUSIONS: We conclude that carnosic acid inhibits IL-1beta-induced ICAM-1, VCAM-1 and E-selectin expression in HUVECs through a mechanism that involves NFkappaB. We propose that the reduction in binding of human monocytic cell line U937 to IL-1beta-treated HUVECs is due to the anti-inflammatory properties of carnosic acid.
Authors: Abdullah A AlKahtane; Esraa Ghanem; Simona G Bungau; Saud Alarifi; Daoud Ali; Gadah AlBasher; Saad Alkahtani; Lotfi Aleya; Mohamed M Abdel-Daim Journal: Environ Sci Pollut Res Int Date: 2020-01-22 Impact factor: 4.223
Authors: Jueun Oh; Tao Yu; Soo Jeong Choi; Yanyan Yang; Heung Soo Baek; Soon Ae An; Lee Kyoung Kwon; Jinsol Kim; Ho Sik Rho; Song Seok Shin; Wahn Soo Choi; Sungyoul Hong; Jae Youl Cho Journal: Mediators Inflamm Date: 2012-04-10 Impact factor: 4.711