Literature DB >> 19141391

Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.

Akira Matsuno1, Johbu Itoh, Akiko Mizutani, Susumu Takekoshi, R Yoshiyuki Osamura, Hiroko Okinaga, Fuyuaki Ide, Satoru Miyawaki, Takeshi Uno, Shuichiro Asano, Junichi Tanaka, Hiroshi Nakaguchi, Mitsuyoshi Sasaki, Mineko Murakami.   

Abstract

Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.

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Year:  2008        PMID: 19141391     DOI: 10.2478/v10042-008-0069-5

Source DB:  PubMed          Journal:  Folia Histochem Cytobiol        ISSN: 0239-8508            Impact factor:   1.698


  5 in total

1.  3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.

Authors:  Sunxi Wang; Jin-Sook Lee; Nicole Bishop; Aleksandar Jeremic; Won Jin Cho; Xuequn Chen; Guangzhao Mao; Douglas J Taatjes; Bhanu P Jena
Journal:  Histochem Cell Biol       Date:  2012-04-13       Impact factor: 4.304

Review 2.  Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion.

Authors:  Akira Matsuno; Akiko Mizutani; Hiroko Okinaga; Koji Takano; So Yamada; Shoko M Yamada; Hiroshi Nakaguchi; Katsumi Hoya; Mineko Murakami; Masato Takeuchi; Mutsumi Sugaya; Johbu Itoh; Susumu Takekoshi; R Yoshiyuki Osamura
Journal:  Med Mol Morphol       Date:  2011-06-30       Impact factor: 2.309

3.  Neuronal Porosome-The Secretory Portal at the Nerve Terminal: It's Structure-Function, Composition, and Reconstitution.

Authors:  Bhanu P Jena
Journal:  J Mol Struct       Date:  2014-09-05       Impact factor: 3.196

4.  SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kgamma Activity through Deacetylation of Specific Lysine Residues in Mammals.

Authors:  Sayaka Akieda-Asai; Nobuhiro Zaima; Koji Ikegami; Tomoaki Kahyo; Ikuko Yao; Takahiro Hatanaka; Shun-Ichiro Iemura; Rika Sugiyama; Takeaki Yokozeki; Yoshinobu Eishi; Morio Koike; Kyoji Ikeda; Takuya Chiba; Haruyoshi Yamaza; Isao Shimokawa; Si-Young Song; Akira Matsuno; Akiko Mizutani; Motoji Sawabe; Moses V Chao; Masashi Tanaka; Yasunori Kanaho; Tohru Natsume; Haruhiko Sugimura; Yukari Date; Michael W McBurney; Leonard Guarente; Mitsutoshi Setou
Journal:  PLoS One       Date:  2010-07-23       Impact factor: 3.240

Review 5.  Molecular morphology of pituitary cells, from conventional immunohistochemistry to fluorescein imaging.

Authors:  Akira Matsuno; Akiko Mizutani; Hiroko Okinaga; Koji Takano; So Yamada; Shoko M Yamada; Hiroshi Nakaguchi; Katsumi Hoya; Mineko Murakami; Masato Takeuchi; Mutsumi Sugaya; Johbu Itoh; Susumu Takekoshi; R Yoshiyuki Osamura
Journal:  Molecules       Date:  2011-04-29       Impact factor: 4.411

  5 in total

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