Literature DB >> 19137092

Immobilized metal affinity electrophoresis: a novel method of capturing phosphoproteins by electrophoresis.

Bao-Shiang Lee1, G D Lasanthi, P Jayathilaka, Jin-Sheng Huang, Shalini Gupta.   

Abstract

An immobilized metal affinity electrophoresis (IMAEP) method is described here. In this method, metal ions are immobilized in a native polyacrylamide gel to capture phosphoproteins. The capture of phosphoproteins by IMAEP is demonstrated with immobilized metals like iron, aluminum, manganese, or titanium. In the case studies, phosphoproteins alpha-casein, beta-casein, and phosvitin are successfully extracted from a protein mixture by IMAEP.

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Year:  2008        PMID： 19137092      PMCID： PMC2361167     

Source DB:  PubMed          Journal:  J Biomol Tech        ISSN： 1524-0215


  8 in total

1.  Mapping the phosphorylation sites of proteins using on-line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization multiple stage tandem mass spectrometry.

Authors:  P Cao; J T Stults
Journal:  Rapid Commun Mass Spectrom       Date:  2000       Impact factor: 2.419

2.  Immobilized metal ion affinity chromatography of proteins.

Authors:  Michael Zachariou
Journal:  Methods Mol Biol       Date:  2004

3.  Determination of protein phosphorylation sites by mass spectrometry: a novel electrospray-based method.

Authors:  Vera Alverdi; Francesca Di Pancrazio; Giovanna Lippe; Carlo Pucillo; Bruno Casetta; Gennaro Esposito
Journal:  Rapid Commun Mass Spectrom       Date:  2005       Impact factor: 2.419

Review 4.  Mapping protein post-translational modifications with mass spectrometry.

Authors:  Eric S Witze; William M Old; Katheryn A Resing; Natalie G Ahn
Journal:  Nat Methods       Date:  2007-10       Impact factor: 28.547

5.  Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography.

Authors:  L Andersson; J Porath
Journal:  Anal Biochem       Date:  1986-04       Impact factor: 3.365

Review 6.  Perspectives of immobilized-metal affinity chromatography.

Authors:  V Gaberc-Porekar; V Menart
Journal:  J Biochem Biophys Methods       Date:  2001-10-30

7.  Evidence for phosphorylation of serine 753 in CFTR using a novel metal-ion affinity resin and matrix-assisted laser desorption mass spectrometry.

Authors:  D C Neville; C R Rozanas; E M Price; D B Gruis; A S Verkman; R R Townsend
Journal:  Protein Sci       Date:  1997-11       Impact factor: 6.725

8.  Dephosphorylation of chicken riboflavin-binding protein and phosvitin decreases their uptake by oocytes.

Authors:  M S Miller; M Benore-Parsons; H B White
Journal:  J Biol Chem       Date:  1982-06-25       Impact factor: 5.157
  8 in total
  2 in total

1.  Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE.

Authors:  Eiji Kinoshita; Emiko Kinoshita-Kikuta; Tohru Koike
Journal:  Nat Protoc       Date:  2009-09-24       Impact factor: 13.491

2.  Two-dimensional immobilized metal affinity electrophoresis for capturing a phosphoprotein.

Authors:  Shalini Gupta; G D Lasanthi; P Jayathilaka; Jin-Sheng Huang; Bao-Shiang Lee
Journal:  J Biomol Tech       Date:  2010-12
  2 in total

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