| Literature DB >> 19133270 |
Alexandra Popova-Butler1, Donald H Dean.
Abstract
We analyzed brush border membrane vesicle proteins from isolated midguts of the mosquito Aedes aegypti, by two proteomic methods: two-dimensional gel electrophoresis (isoelectric focusing and SDS-PAGE) and a shotgun two-dimensional liquid chromatographic (LS/LS) approach based on multidimensional protein identification technology (MudPIT). We were interested in the most abundant proteins of the apical brush border midgut membrane. About 400 spots were detected on 2D gels and 39 spots were cored and identified by mass spectrometry. 86 proteins were identified by MudPIT. Three proteins, arginine kinase, putative allergen and actin are shown to be the most predominant proteins in the sample. The total number of 36 proteins detected by both methods represents the most abundant proteins in the BBMV.Entities:
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Year: 2009 PMID： 19133270 PMCID： PMC2735124 DOI： 10.1016/j.jinsphys.2008.12.008
Source DB: PubMed Journal: J Insect Physiol ISSN： 0022-1910 Impact factor: 2.354