BACKGROUND: In this study we sought to validate urinary biomarkers for diabetes and two common complications, coronary artery disease (CAD) and diabetic nephropathy (DN). METHODS: A CAD score calculated by summing the product of a classification coefficient and signal amplitude of 15 urinary polypeptides was previously developed. Five sequences of biomarkers in the panel were identified as fragments of collagen alpha-1(I) and alpha-1(III). Prospectively collected urine samples available for analysis from 19 out of 20 individuals with CAD (15 with type 1 diabetes [T1D] and four without diabetes) and age-, sex-, and diabetes-matched controls enrolled in the Coronary Artery Calcification in Type 1 Diabetes study were analyzed for the CAD score using capillary electrophoresis and electrospray ionization mass spectrometry. Two panels of biomarkers that were previously defined to distinguish diabetes status were analyzed to determine their relationship to T1D. Three biomarker panels developed to distinguish DN (DNS) and two biomarker panels developed to distinguish renal disease (RDS) were examined to determine their relationship with renal function. RESULTS: The CAD score was associated with CAD (odds ratio with 95% confidence interval, 2.2 [1.3-5.2]; P = 0.0016) and remained significant when adjusted individually for age, albumin excretion rate (AER), blood pressure, waist circumference, intraabdominal fat, glycosylated hemoglobin, and lipids. DNS and RDS were significantly correlated with AER, cystatin C, and serum creatinine. The biomarker panels for diabetes were both significantly associated with T1D status (P < 0.05 for both). CONCLUSIONS: We validated a urinary proteome pattern associated with CAD and urinary proteome patterns associated with T1D and DN.
BACKGROUND: In this study we sought to validate urinary biomarkers for diabetes and two common complications, coronary artery disease (CAD) and diabetic nephropathy (DN). METHODS: A CAD score calculated by summing the product of a classification coefficient and signal amplitude of 15 urinary polypeptides was previously developed. Five sequences of biomarkers in the panel were identified as fragments of collagen alpha-1(I) and alpha-1(III). Prospectively collected urine samples available for analysis from 19 out of 20 individuals with CAD (15 with type 1 diabetes [T1D] and four without diabetes) and age-, sex-, and diabetes-matched controls enrolled in the Coronary Artery Calcification in Type 1 Diabetes study were analyzed for the CAD score using capillary electrophoresis and electrospray ionization mass spectrometry. Two panels of biomarkers that were previously defined to distinguish diabetes status were analyzed to determine their relationship to T1D. Three biomarker panels developed to distinguish DN (DNS) and two biomarker panels developed to distinguish renal disease (RDS) were examined to determine their relationship with renal function. RESULTS: The CAD score was associated with CAD (odds ratio with 95% confidence interval, 2.2 [1.3-5.2]; P = 0.0016) and remained significant when adjusted individually for age, albumin excretion rate (AER), blood pressure, waist circumference, intraabdominal fat, glycosylated hemoglobin, and lipids. DNS and RDS were significantly correlated with AER, cystatin C, and serum creatinine. The biomarker panels for diabetes were both significantly associated with T1D status (P < 0.05 for both). CONCLUSIONS: We validated a urinary proteome pattern associated with CAD and urinary proteome patterns associated with T1D and DN.
Authors: Stefan Schaub; John Wilkins; Tracey Weiler; Kevin Sangster; David Rush; Peter Nickerson Journal: Kidney Int Date: 2004-01 Impact factor: 10.612
Authors: David M Good; Visith Thongboonkerd; Jan Novak; Jean-Loup Bascands; Joost P Schanstra; Joshua J Coon; Anna Dominiczak; Harald Mischak Journal: J Proteome Res Date: 2007-10-31 Impact factor: 4.466
Authors: Chris F Taylor; Norman W Paton; Kathryn S Lilley; Pierre-Alain Binz; Randall K Julian; Andrew R Jones; Weimin Zhu; Rolf Apweiler; Ruedi Aebersold; Eric W Deutsch; Michael J Dunn; Albert J R Heck; Alexander Leitner; Marcus Macht; Matthias Mann; Lennart Martens; Thomas A Neubert; Scott D Patterson; Peipei Ping; Sean L Seymour; Puneet Souda; Akira Tsugita; Joel Vandekerckhove; Thomas M Vondriska; Julian P Whitelegge; Marc R Wilkins; Ioannnis Xenarios; John R Yates; Henning Hermjakob Journal: Nat Biotechnol Date: 2007-08 Impact factor: 54.908
Authors: Won Yong Kim; Anne Sofie Astrup; Matthias Stuber; Lise Tarnow; Erling Falk; René M Botnar; Cheryl Simonsen; Lotte Pietraszek; Peter R Hansen; Warren J Manning; Niels T Andersen; Hans-Henrik Parving Journal: Circulation Date: 2006-12-26 Impact factor: 29.690
Authors: Kasper Rossing; Harald Mischak; Hans-Henrik Parving; Per K Christensen; Michael Walden; Meike Hillmann; Thorsten Kaiser Journal: Kidney Int Date: 2005-07 Impact factor: 10.612
Authors: Lukas U Zimmerli; Eric Schiffer; Petra Zürbig; David M Good; Markus Kellmann; Laetitia Mouls; Andrew R Pitt; Joshua J Coon; Roland E Schmieder; Karlheinz H Peter; Harald Mischak; Walter Kolch; Christian Delles; Anna F Dominiczak Journal: Mol Cell Proteomics Date: 2007-10-19 Impact factor: 5.911
Authors: Dan Theodorescu; Stefan Wittke; Mark M Ross; Michael Walden; Mark Conaway; Ingo Just; Harald Mischak; Henry F Frierson Journal: Lancet Oncol Date: 2006-03 Impact factor: 41.316
Authors: Nils v Neuhoff; Thorsten Kaiser; Stefan Wittke; Ronald Krebs; Andrew Pitt; Aurea Burchard; Astrid Sundmacher; Brigitte Schlegelberger; Walter Kolch; Harald Mischak Journal: Rapid Commun Mass Spectrom Date: 2004 Impact factor: 2.419
Authors: Stephane Decramer; Stefan Wittke; Harald Mischak; Petra Zürbig; Michael Walden; François Bouissou; Jean-Loup Bascands; Joost P Schanstra Journal: Nat Med Date: 2006-03-19 Impact factor: 53.440
Authors: Harald Mischak; Walter Kolch; Michalis Aivaliotis; David Bouyssié; Magali Court; Hassan Dihazi; Gry H Dihazi; Julia Franke; Jérôme Garin; Anne Gonzalez de Peredo; Alexander Iphöfer; Lothar Jänsch; Chrystelle Lacroix; Manousos Makridakis; Christophe Masselon; Jochen Metzger; Bernard Monsarrat; Michal Mrug; Martin Norling; Jan Novak; Andreas Pich; Andrew Pitt; Erik Bongcam-Rudloff; Justyna Siwy; Hitoshi Suzuki; Visith Thongboonkerd; Li-Shun Wang; Jérôme Zoidakis; Petra Zürbig; Joost P Schanstra; Antonia Vlahou Journal: Proteomics Clin Appl Date: 2010-02-03 Impact factor: 3.494
Authors: David M Maahs; Justyna Siwy; Angel Argilés; Marie Cerna; Christian Delles; Anna F Dominiczak; Nathalie Gayrard; Alexander Iphöfer; Lothar Jänsch; George Jerums; Karel Medek; Harald Mischak; Gerjan J Navis; Johannes M Roob; Kasper Rossing; Peter Rossing; Ivan Rychlík; Eric Schiffer; Roland E Schmieder; Thomas C Wascher; Brigitte M Winklhofer-Roob; Lukas U Zimmerli; Petra Zürbig; Janet K Snell-Bergeon Journal: PLoS One Date: 2010-09-28 Impact factor: 3.240