Yunlong Li1, Da Xing, Qun Chen. 1. MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, 510631, China.
Abstract
PURPOSE: The aim of the study is to dynamically and non-invasively monitor the temporal relationship among caspase-3, BID, and cytochrome c in chemotherapy. PROCEDURES: ASTC-a-1 cells expressing the corresponding fluorescence reporters were treated with Taxol or cisplatin and imaged using FRET and fluorescence overlapping technique. Western blot was performed to validate the fluorescence analysis. RESULTS: In fluorescence imaging analysis, Taxol-induced apoptosis showed caspase-3 activation (13 h 50 min) was prior to BID cleavage (15 h 10 min) and subsequent significant cytochrome c release (17-18 h 20 min), whereas the cisplatin-induced apoptosis showed BID cleavage (5 h 40 min) and significant cytochrome c release (7-8 h 20 min) were prior to caspase-3 activation (14 h 20 min). Western blot further validated the results above. CONCLUSIONS: The new approach successfully reveals the difference in temporal signaling apoptosis events between Taxol and cisplatin. It may help us come to a better understanding of the detailed mechanisms in chemotherapeutic-agents-induced apoptosis.
PURPOSE: The aim of the study is to dynamically and non-invasively monitor the temporal relationship among caspase-3, BID, and cytochrome c in chemotherapy. PROCEDURES: ASTC-a-1 cells expressing the corresponding fluorescence reporters were treated with Taxol or cisplatin and imaged using FRET and fluorescence overlapping technique. Western blot was performed to validate the fluorescence analysis. RESULTS: In fluorescence imaging analysis, Taxol-induced apoptosis showed caspase-3 activation (13 h 50 min) was prior to BID cleavage (15 h 10 min) and subsequent significant cytochrome c release (17-18 h 20 min), whereas the cisplatin-induced apoptosis showed BID cleavage (5 h 40 min) and significant cytochrome c release (7-8 h 20 min) were prior to caspase-3 activation (14 h 20 min). Western blot further validated the results above. CONCLUSIONS: The new approach successfully reveals the difference in temporal signaling apoptosis events between Taxol and cisplatin. It may help us come to a better understanding of the detailed mechanisms in chemotherapeutic-agents-induced apoptosis.
Authors: Clarissa von Haefen; Thomas Wieder; Frank Essmann; Klaus Schulze-Osthoff; Bernd Dörken; Peter T Daniel Journal: Oncogene Date: 2003-04-17 Impact factor: 9.867
Authors: Michael Jelínek; Kamila Balušíková; Martina Schmiedlová; Vlasta Němcová-Fürstová; Jan Šrámek; Jitka Stančíková; Ilaria Zanardi; Iwao Ojima; Jan Kovář Journal: Cancer Cell Int Date: 2015-02-04 Impact factor: 5.722