BACKGROUND AND PURPOSE: MIF has been implicated to function in many inflammatory processes. This study examined whether MIF expression was affected in stroke and its underlying molecular mechanism. METHODS: ELISA and qRT-PCR were used to detect MIF protein and mRNA in PBMCs from stroke patients, the ischemic rat brains, and controls. A MIF promoter assay under hypoxia was performed. RESULTS: MIF protein and mRNA were significantly increased in stroke patients. Increasing levels of MIF were correlated to the severity of stroke and peaked 24 hours after stroke. MIF was significantly upregulated in focal ischemic rat brains. The activity of the human MIF promoter was significantly increased under hypoxia compared to normoxia. CONCLUSIONS: MIF gene expression is upregulated after stroke, and hypoxia signaling plays an important role in upregulation of MIF expression under stroke.
BACKGROUND AND PURPOSE:MIF has been implicated to function in many inflammatory processes. This study examined whether MIF expression was affected in stroke and its underlying molecular mechanism. METHODS: ELISA and qRT-PCR were used to detect MIF protein and mRNA in PBMCs from strokepatients, the ischemicrat brains, and controls. A MIF promoter assay under hypoxia was performed. RESULTS:MIF protein and mRNA were significantly increased in strokepatients. Increasing levels of MIF were correlated to the severity of stroke and peaked 24 hours after stroke. MIF was significantly upregulated in focal ischemicrat brains. The activity of the humanMIF promoter was significantly increased under hypoxia compared to normoxia. CONCLUSIONS:MIF gene expression is upregulated after stroke, and hypoxia signaling plays an important role in upregulation of MIF expression under stroke.
Authors: Amanda Ellis; Julie Wieseler; Jacob Favret; Kirk W Johnson; Kenner C Rice; Steven F Maier; Scott Falci; Linda R Watkins Journal: J Pain Date: 2014-01-09 Impact factor: 5.820
Authors: Ana R Inácio; Karsten Ruscher; Lin Leng; Richard Bucala; Tomas Deierborg Journal: J Cereb Blood Flow Metab Date: 2010-11-10 Impact factor: 6.200
Authors: Si Zhang; Odysseus Zis; Philip T T Ly; Yili Wu; Shuting Zhang; Mingming Zhang; Fang Cai; Richard Bucala; Woei-Cherng Shyu; Weihong Song Journal: FASEB J Date: 2014-06-26 Impact factor: 5.191