Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.

Literature DB >> 19123774

User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.

Hendrik Schroeder¹, Michael Adler, Katrin Gerigk, Birgit Müller-Chorus, Friedrich Götz, Christof M Niemeyer.

Abstract

We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.

Entities: Chemical

Mesh：

Substances：

Year: 2009 PMID： 19123774 DOI： 10.1021/ac802228k

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

Keyword Cloud
Cited

10 in total

Review 1. Protein immobilization techniques for microfluidic assays.

Authors: Dohyun Kim; Amy E Herr
Journal: Biomicrofluidics Date: 2013-07-30 Impact factor: 2.800

2. Sensitive and continuous screening of inhibitors of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) at single SPR chips.

Authors: Xinyao Yi; Yuanqiang Hao; Ning Xia; Jianxiu Wang; Monica Quintero; Ding Li; Feimeng Zhou
Journal: Anal Chem Date: 2013-03-11 Impact factor: 6.986

3. DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format.

Authors: Adam L Washburn; Joseph Gomez; Ryan C Bailey
Journal: Anal Chem Date: 2011-04-06 Impact factor: 6.986

4. Chemistries for patterning robust DNA microbarcodes enable multiplex assays of cytoplasm proteins from single cancer cells.

Authors: Young Shik Shin; Habib Ahmad; Qihui Shi; Hyungjun Kim; Tod A Pascal; Rong Fan; William A Goddard; James R Heath
Journal: Chemphyschem Date: 2010-10-04 Impact factor: 3.102

5. Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA).

Authors: Ziyi He; Justin Huffman; Kathrine Curtin; Krista L Garner; Elizabeth C Bowdridge; Xiaojun Li; Timothy R Nurkiewicz; Peng Li
Journal: Anal Chem Date: 2020-12-16 Impact factor: 6.986

User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.

Review 1. Protein immobilization techniques for microfluidic assays.

2. Sensitive and continuous screening of inhibitors of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) at single SPR chips.

3. DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format.

4. Chemistries for patterning robust DNA microbarcodes enable multiplex assays of cytoplasm proteins from single cancer cells.

5. Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA).

6. Selective covalent conjugation of phosphorothioate DNA oligonucleotides with streptavidin.

Review 7. Opto-Microfluidic Immunosensors: From Colorimetric to Plasmonic.

Review 8. Miniaturized technology for protein and nucleic acid point-of-care testing.

9. Oligonucleotide-peptide conjugates: solid-phase synthesis under acidic conditions and use in ELISA assays.

Review 10. Applications of protein biochips in biomedical and biotechnological research.