J Li1, Z Liu, Y Wu, H Wu, P Ran. 1. Allergy and Immunology Institute, Shenzhen University School of Medicine, Shenzhen University, Shenzhen, China.
Abstract
BACKGROUND: In recent years, nanoparticle materials have found wide applications in drug transport and release systems. Chitosan is a good carrier for proteins, peptides, and nucleic acids because of its favorable release properties and ability to increase membrane permeability. OBJECTIVE: To investigate the effect of chitosan microparticles loaded with the major epitope peptide of mite group 2 allergen Der f 2 from Dermatophagoides farinae (Der f 2-47-67) in alleviating asthma in mice. METHODS: Derf 2-47-67 was entrapped in chitosan to obtain Derf 2-47-67-loaded chitosan microparticles, which were injected intraperitoneally into BALB/c mice prior to an intranasal challenge with a Der f extract allergen. Airway hyperreactivity was measured via whole-body plethysmography, and bronchoalveolar lavage (BAL) fluid was collected to calculate total cell and eosinophil counts. Changes in lung histology were assessed after hematoxylin-eosin staining, and serum levels of Der f-specific immunoglobulin (Ig) G2a and IgE were determined by enzyme-linked immunoabsorbent assay. RESULTS: Mice immunized with Der f 2-47-67-loaded chitosan microparticles displayed decreased airway hyperreactivity, reduced numbers of eosinophils in BAL fluid, alleviated lung inflammation and mucus production, a reduced serum level of Der f-specific IgE and an increased serum level of Der f-specific IgG2a. CONCLUSION: Our data showed that Der f 2-47-67-loaded chitosan microparticles inhibited airway allergic inflammation.
BACKGROUND: In recent years, nanoparticle materials have found wide applications in drug transport and release systems. Chitosan is a good carrier for proteins, peptides, and nucleic acids because of its favorable release properties and ability to increase membrane permeability. OBJECTIVE: To investigate the effect of chitosan microparticles loaded with the major epitope peptide of mite group 2 allergen Der f 2 from Dermatophagoides farinae (Der f 2-47-67) in alleviating asthma in mice. METHODS: Derf 2-47-67 was entrapped in chitosan to obtain Derf 2-47-67-loaded chitosan microparticles, which were injected intraperitoneally into BALB/c mice prior to an intranasal challenge with a Der f extract allergen. Airway hyperreactivity was measured via whole-body plethysmography, and bronchoalveolar lavage (BAL) fluid was collected to calculate total cell and eosinophil counts. Changes in lung histology were assessed after hematoxylin-eosin staining, and serum levels of Der f-specific immunoglobulin (Ig) G2a and IgE were determined by enzyme-linked immunoabsorbent assay. RESULTS:Mice immunized with Der f 2-47-67-loaded chitosan microparticles displayed decreased airway hyperreactivity, reduced numbers of eosinophils in BAL fluid, alleviated lung inflammation and mucus production, a reduced serum level of Der f-specific IgE and an increased serum level of Der f-specific IgG2a. CONCLUSION: Our data showed that Der f 2-47-67-loaded chitosan microparticles inhibited airway allergic inflammation.
Authors: Michael R Shurin; Naveena Yanamala; Elena R Kisin; Alexey V Tkach; Galina V Shurin; Ashley R Murray; Howard D Leonard; Jeffrey S Reynolds; Dmirtiy W Gutkin; Alexander Star; Bengt Fadeel; Kai Savolainen; Valerian E Kagan; Anna A Shvedova Journal: ACS Nano Date: 2014-05-23 Impact factor: 15.881