Literature DB >> 19122859

Development of analytical methods for multiplex bio-assay with inductively coupled plasma mass spectrometry.

Olga I Ornatsky1, Robert Kinach, Dmitry R Bandura, Xudong Lou, Scott D Tanner, Vladimir I Baranov, Mark Nitz, Mitchell A Winnik.   

Abstract

Advances in the development of highly multiplexed bio-analytical assays with inductively coupled plasma mass spectrometry (ICP-MS) detection are discussed. Use of novel reagents specifically designed for immunological methods utilizing elemental analysis is presented. The major steps of method development, including selection of elements for tags, validation of tagged reagents, and examples of multiplexed assays, are considered in detail. The paper further describes experimental protocols for elemental tagging of antibodies, immunostaining of live and fixed human leukemia cells, and preparation of samples for ICP-MS analysis. Quantitative analysis of surface antigens on model cell lines using a cocktail of seven lanthanide labeled antibodies demonstrated high specificity and concordance with conventional immunophenotyping.

Entities:  

Year:  2008        PMID: 19122859      PMCID: PMC2600572          DOI: 10.1039/b710510j

Source DB:  PubMed          Journal:  J Anal At Spectrom        ISSN: 0267-9477            Impact factor:   4.023


  26 in total

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