PURPOSE: To report the immunosuppressive property of dry human amniotic membrane (dHAM). METHODS: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. RESULTS: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). CONCLUSION: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay. (c) 2008 S. Karger AG, Basel.
PURPOSE: To report the immunosuppressive property of dry human amniotic membrane (dHAM). METHODS:Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. RESULTS: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). CONCLUSION: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay. (c) 2008 S. Karger AG, Basel.
Authors: Michelle Massee; Kathryn Chinn; Jennifer Lei; Jeremy J Lim; Conan S Young; Thomas J Koob Journal: J Biomed Mater Res B Appl Biomater Date: 2015-07-14 Impact factor: 3.368
Authors: Alexandra M Haugh; Jacqueline G Witt; Adam Hauch; Michael Darden; Geoffrey Parker; Warren A Ellsworth; Joseph F Buell Journal: Plast Reconstr Surg Glob Open Date: 2017-04-25