Bioactive constituents from Chinese natural medicines. XXXIII. Inhibitors from the seeds of Psoralea corylifolia on production of nitric oxide in lipopolysaccharide-activated macrophages.

The methanolic extract from the seeds of Psoralea corylifolia was found to inhibit production of nitric oxide (NO) in lipopolysaccharide-activated mouse peritoneal macrophages. Among the isolated compounds, bavachinin (IC(50)=26 microM), isobavachalcone (17 microM), neobavaisoflavone (ca. 29 microM), corylifol A (ca. 21 microM), and psoralidin (ca. 23 microM) significantly inhibited the accumulation of nitrite (NO(2)(-)) as a marker of production of NO. Bakuchiol, which is mainly contained in the extract, also showed weak activity at 10 microM, but cytotoxic effects were observed more than 30 microM.