AIMS: To identify and characterize bacteriophages specific for Edwardsiella ictaluri, the causative agent for enteric septicemia of catfish (ESC). METHODS AND RESULTS: Two bacteriophages were isolated that infect Edw. ictaluri. They both produce clear plaques, have icosahedral heads with a non-rigid tail, and are tentatively classified as Siphoviridae. Phages PhieiDWF and PhieiAU are dsDNA viruses with approximate genome sizes of 40 and 45 kb, respectively. The addition of 500 micromol l(-1) CaCl(2) enhanced phage titres. Both phages have a latent period of 40 min and an estimated burst size of 270. Every Edw. ictaluri strain tested was susceptible to phage infection with variable plaquing efficiencies and with no evidence of lysogeny, with no plaques detected on other bacterial species. CONCLUSIONS: Two unique bacteriophages were isolated that show host-specificity for Edw. ictaluri, have temperature and metal cation-dependent infectivity, and are tentatively placed within the family Siphoviridae. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of bacteriophages specific to Edw. ictaluri, an important fish pathogen affecting farm-raised channel catfish. Initial characterization of these bacteriophages has demonstrated their potential use as biotherapeutic and diagnostic agents associated with ESC.
AIMS: To identify and characterize bacteriophages specific for Edwardsiella ictaluri, the causative agent for enteric septicemia of catfish (ESC). METHODS AND RESULTS: Two bacteriophages were isolated that infect Edw. ictaluri. They both produce clear plaques, have icosahedral heads with a non-rigid tail, and are tentatively classified as Siphoviridae. Phages PhieiDWF and PhieiAU are dsDNA viruses with approximate genome sizes of 40 and 45 kb, respectively. The addition of 500 micromol l(-1) CaCl(2) enhanced phage titres. Both phages have a latent period of 40 min and an estimated burst size of 270. Every Edw. ictaluri strain tested was susceptible to phage infection with variable plaquing efficiencies and with no evidence of lysogeny, with no plaques detected on other bacterial species. CONCLUSIONS: Two unique bacteriophages were isolated that show host-specificity for Edw. ictaluri, have temperature and metal cation-dependent infectivity, and are tentatively placed within the family Siphoviridae. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of bacteriophages specific to Edw. ictaluri, an important fish pathogen affecting farm-raised channel catfish. Initial characterization of these bacteriophages has demonstrated their potential use as biotherapeutic and diagnostic agents associated with ESC.
Authors: Adelaide Almeida; Angela Cunha; Newton C M Gomes; Eliana Alves; Liliana Costa; Maria A F Faustino Journal: Mar Drugs Date: 2009-07-30 Impact factor: 5.118
Authors: Abel Carrias; Timothy J Welch; Geoffrey C Waldbieser; David A Mead; Jeffery S Terhune; Mark R Liles Journal: Virol J Date: 2011-01-07 Impact factor: 4.099
Authors: Tuan Son Le; Thi Hien Nguyen; Hong Phuong Vo; Van Cuong Doan; Hong Loc Nguyen; Minh Trung Tran; Trong Tuan Tran; Paul C Southgate; D İpek Kurtböke Journal: Antibiotics (Basel) Date: 2018-02-25