AIMS: To study the distribution of norovirus (NV) in oyster tissues. METHODS AND RESULTS: Five monoclonal antibodies (MAbs) against VP1 were selected from Balb/c mice. Immunohistochemical analysis was performed to detect native NV in different tissues of artificially contaminated oysters using the MAbs. The data showed that the gills and the digestive glands are efficient tissues for accumulation of the NV. In addition, the NV was found on the cilia of the mantle after filtering. In our study, only NV RNA in the gills could be detected by reverse transcriptase-polymerase chain reaction. CONCLUSIONS: NV was bioaccumulated in the gills, stomach, digestive diverticula and cilia of the mantle. Furthermore, the results suggested that the viral load of the gills and the digestive glands is heavier than that of the other tissues. SIGNIFICANCE AND IMPACT OF THE STUDY: This, to our knowledge, is the first paper to report the distribution of NV in oyster tissues by immunoassay after artificial contamination. Further understanding of the NV distribution in oyster may help us to sample appropriate tissues for detection of the virus.
AIMS: To study the distribution of norovirus (NV) in oyster tissues. METHODS AND RESULTS: Five monoclonal antibodies (MAbs) against VP1 were selected from Balb/c mice. Immunohistochemical analysis was performed to detect native NV in different tissues of artificially contaminated oysters using the MAbs. The data showed that the gills and the digestive glands are efficient tissues for accumulation of the NV. In addition, the NV was found on the cilia of the mantle after filtering. In our study, only NV RNA in the gills could be detected by reverse transcriptase-polymerase chain reaction. CONCLUSIONS: NV was bioaccumulated in the gills, stomach, digestive diverticula and cilia of the mantle. Furthermore, the results suggested that the viral load of the gills and the digestive glands is heavier than that of the other tissues. SIGNIFICANCE AND IMPACT OF THE STUDY: This, to our knowledge, is the first paper to report the distribution of NV in oyster tissues by immunoassay after artificial contamination. Further understanding of the NV distribution in oyster may help us to sample appropriate tissues for detection of the virus.
Authors: Keleigh Provost; Brooke A Dancho; Gulnihal Ozbay; Robert S Anderson; Gary P Richards; David H Kingsley Journal: Appl Environ Microbiol Date: 2011-09-23 Impact factor: 4.792
Authors: Haifa Maalouf; Julien Schaeffer; Sylvain Parnaudeau; Jacques Le Pendu; Robert L Atmar; Sue E Crawford; Françoise S Le Guyader Journal: Appl Environ Microbiol Date: 2011-03-25 Impact factor: 4.792
Authors: Maha Zakhour; Haifa Maalouf; Ilaria Di Bartolo; Larissa Haugarreau; Françoise S Le Guyader; Nathalie Ruvoën-Clouet; Jean-Claude Le Saux; Franco Maria Ruggeri; Monique Pommepuy; Jacques Le Pendu Journal: Appl Environ Microbiol Date: 2010-08-13 Impact factor: 4.792
Authors: Françoise S Le Guyader; Joanna Krol; Katia Ambert-Balay; Nathalie Ruvoen-Clouet; Benedicte Desaubliaux; Sylvain Parnaudeau; Jean-Claude Le Saux; Agnès Ponge; Pierre Pothier; Robert L Atmar; Jacques Le Pendu Journal: J Clin Microbiol Date: 2010-01-06 Impact factor: 5.948