Literature DB >> 1911849

A fluorometric study with 1-anilinonaphthalene-8-sulfonic acid (ANS) of the interactions of ATP and ADP with rubisco activase.

Z Y Wang1, A R Portis.   

Abstract

The interactions of ATP and ADP with rubisco activase purified from spinach were investigated by measuring enhanced fluorescence due to ANS-binding to the protein. Evidence of conformational changes was observed from the differences in the interaction of ANS with rubisco activase in the presence of excess ATP and ADP. Fluorescent changes associated with the titration of a rubisco activase-ANS mixture with ATP and ADP indicated that the binding of ADP to rubisco activase was much tighter than that of ATP. The concentration of Mg2+ and pH had significant effects on the affinities of rubisco activase for ATP and ADP, with higher pH and Mg2+ concentration facilitating the binding of ATP to rubisco activase in the presence of ADP. The physiological implications of the binding characteristics of ATP and ADP with rubisco activase on the light-dark regulation of rubisco are discussed.

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Year:  1991        PMID： 1911849     DOI： 10.1016/0167-4838(91)90067-a

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  8 in total

1.  Alteration of the adenine nucleotide response and increased Rubisco activation activity of Arabidopsis rubisco activase by site-directed mutagenesis.

Authors:  R P Kallis; R G Ewy; A R Portis
Journal:  Plant Physiol       Date:  2000-07       Impact factor: 8.340

2.  NanoESI mass spectrometry of Rubisco and Rubisco activase structures and their interactions with nucleotides and sugar phosphates.

Authors:  Michelle J Blayney; Spencer M Whitney; Jennifer L Beck
Journal:  J Am Soc Mass Spectrom       Date:  2011-06-29       Impact factor: 3.109

3.  The mechanism of Rubisco activase: Insights from studies of the properties and structure of the enzyme.

Authors:  M E Salvucci; W L Ogren
Journal:  Photosynth Res       Date:  1996-01       Impact factor: 3.573

4.  A single point mutation in the C-terminal extension of wheat Rubisco activase dramatically reduces ADP inhibition via enhanced ATP binding affinity.

Authors:  Andrew P Scafaro; David De Vleesschauwer; Nadine Bautsoens; Matthew A Hannah; Bart den Boer; Alexander Gallé; Jeroen Van Rie
Journal:  J Biol Chem       Date:  2019-09-17       Impact factor: 5.157

5.  Two conserved tryptophan residues are responsible for intrinsic fluorescence enhancement in Rubisco activase upon ATP binding.

Authors:  Dafu Wang; Archie R Portis
Journal:  Photosynth Res       Date:  2006-04-29       Impact factor: 3.573

6.  Species-dependent variation in the interaction of substrate-bound ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) and rubisco activase.

Authors:  Z Y Wang; G W Snyder; B D Esau; A R Portis; W L Ogren
Journal:  Plant Physiol       Date:  1992-12       Impact factor: 8.340

7.  Regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase: product inhibition, cooperativity, and magnesium activation.

Authors:  Suratna Hazra; J Nathan Henderson; Kevin Liles; Matthew T Hilton; Rebekka M Wachter
Journal:  J Biol Chem       Date:  2015-08-17       Impact factor: 5.157

8.  Reversible association of ribulose-1, 5-bisphosphate carboxylase/oxygenase activase with the thylakoid membrane depends upon the ATP level and pH in rice without heat stress.

Authors:  Juan Chen; Peng Wang; Hua-Ling Mi; Gen-Yun Chen; Da-Quan Xu
Journal:  J Exp Bot       Date:  2010-05-17       Impact factor: 6.992
  8 in total

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