Demonstration of different glycosylated antigens in C-type virus-transformed and infected rat cells by antiserum to murine leukemia virus.

[3H]Glucosamine labeling of untransformed cells, C-type virus-transformed cells, and virus-infected cells and subsequent analysis by polyacrylamide gel electrophoresis and fluorography permitted the detection of a Pronase-sensitive macromolecular labeling that appeared in about eight regions of radioactivity in every case. Reactions of cell extracts with antiserum to Tween-ether-disrupted purified murine leukemia virus revealed, in most transformed cells, two components with a mobility of about 100,000 daltons, whereas C-type virus-infected cells revealed their radioactivity mainly in a region nearer to that of the major viral glycoprotein at about 69,000 daltons. No comparable components were apparent from the reaction of transformed or infected extracts with preimmune serum or from the reaction of untransformed uninfected cells and immune serum.