| Literature DB >> 19117681 |
Qiuyue Wang1, Jianhua Li, Xichen Zhang, Quan Liu, Chengwu Liu, Guangpeng Ma, Lili Cao, Pengtao Gong, Yanan Cai, Guocai Zhang.
Abstract
Two recombinant Mycobacterium bovis BCG (rBCG) strains carrying the Eimeria tenella rhomboid gene (Rho) delivered by extrachromosomal vector pMV261 and integrative vector pMV361 were evaluated for their ability to protect chickens against E. tenella challenge. The chickens were immunized intranasal with BCG, rBCG pMV261-Rho, or rBCG pMV361-Rho twice at a 2-week interval. All the recombinant BCG immunized chickens developed specific immune responses, and there was a significant increases of the percentages of CD4(+) and CD8(+) cells compared to the control (P<0.05). Challenge experiments demonstrated that the two rBCG strains could provide significant protection against E. tenella challenge. But vaccination with rBCG pMV261-Rho induced higher specific antibody titers and produced greater protection rate (56.04%) than rBCG pMV361-Rho group (P<0.05). These results indicated that M. bovis BCG is a novel vaccine vector to express and present antigens of E. tenella, and rBCG has a potential as vaccine in chickens.Entities:
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Year: 2008 PMID： 19117681 DOI： 10.1016/j.vetpar.2008.11.006
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738