| Literature DB >> 19117146 |
Carol D Curtis1, Ann M Nardulli.
Abstract
RNA interference can be extremely useful in determining the function of an endogenously-expressed protein in its normal cellular environment. In this chapter, we describe a method that uses small interfering RNA (siRNA) to knock down mRNA and protein expression in cultured cells so that the effect of a putative regulatory protein on gene expression can be delineated. Methods of assessing the effectiveness of the siRNA procedure using real time quantitative PCR and Western analysis are also included.Entities:
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Year: 2009 PMID： 19117146 PMCID： PMC3900309 DOI： 10.1007/978-1-60327-575-0_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745