S Zhang1, X Cai, F Huang, W Zhong, Z Yu. 1. Department of Gastroenterology, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. shinengz@hotmail.com
Abstract
AIM: To investigate the influence of trichostatin A (TSA) on inhibition of cell proliferation and induction of apoptosis in human pancreatic cancer cells. METHODS: MTT-based cytotoxicity assay was used to evaluate the cell viability after treatment with TSA. Cell cycle distribution and apoptosis were examined by means of flow cytometry. Expression of microRNA was determined with microRNA array. Expression of miR-200c and miR-21 was detected by Northern blotting. RESULTS: TSA significantly inhibited the proliferation of BxPC-3 human pancreatic cancer cells in a time- and dose-dependent manner. BxPC-3 cells treated with TSA were arrested in G0/G1 phase and were characterized by increased apoptotic rate, accompanied by differential expression of microRNAs. CONCLUSIONS: The results suggest that TSA may activate expression of microRNAs that may act as tumor suppressor in human pancreatic cancer cell line BxPC-3.
AIM: To investigate the influence of trichostatin A (TSA) on inhibition of cell proliferation and induction of apoptosis in humanpancreatic cancer cells. METHODS:MTT-based cytotoxicity assay was used to evaluate the cell viability after treatment with TSA. Cell cycle distribution and apoptosis were examined by means of flow cytometry. Expression of microRNA was determined with microRNA array. Expression of miR-200c and miR-21 was detected by Northern blotting. RESULTS:TSA significantly inhibited the proliferation of BxPC-3 humanpancreatic cancer cells in a time- and dose-dependent manner. BxPC-3 cells treated with TSA were arrested in G0/G1 phase and were characterized by increased apoptotic rate, accompanied by differential expression of microRNAs. CONCLUSIONS: The results suggest that TSA may activate expression of microRNAs that may act as tumor suppressor in humanpancreatic cancer cell line BxPC-3.
Authors: Jong Y Park; James Helm; Domenico Coppola; Donghwa Kim; Mokenge Malafa; Seung Joon Kim Journal: World J Gastroenterol Date: 2011-02-21 Impact factor: 5.742
Authors: Lyndsay V Rhodes; Ashley M Nitschke; H Chris Segar; Elizabeth C Martin; Jennifer L Driver; Steven Elliott; Seung Yoon Nam; Meng Li; Kenneth P Nephew; Matthew E Burow; Bridgette M Collins-Burow Journal: Oncol Rep Date: 2011-10-04 Impact factor: 3.906