Literature DB >> 19110618

Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials.

Tulin Budak-Alpdogan1, Isabelle Rivière.   

Abstract

This chapter provides information about the oncoretroviral transduction of human hematopoietic stem/ progenitor cells under clinically applicable conditions. We describe in detail a short -60 h transduction protocol which consistently yields transduction efficiencies in the range of 30-50% with five different oncoretroviral vectors. We discuss a number of parameters that affect transduction efficiency, including the oncoretroviral vector characteristics, the vector stock collection, the source of CD34+ cells and transduction conditions.

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Year:  2009        PMID： 19110618      PMCID： PMC4360985          DOI： 10.1007/978-1-59745-409-4_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  98 in total

1.  Expansion of human cord blood CD34(+)CD38(-) cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency: dissociation of SRC phenotype and function.

Authors:  C Dorrell; O I Gan; D S Pereira; R G Hawley; J E Dick
Journal:  Blood       Date:  2000-01-01       Impact factor: 22.113

2.  Determination of infectious retrovirus concentration from colony-forming assay with quantitative analysis.

Authors:  Young Jik Kwon; Gene Hung; W French Anderson; Ching-An Peng; Hong Yu
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103

3.  Efficient retrovirus-mediated transfer of the multidrug resistance 1 gene into autologous human long-term repopulating hematopoietic stem cells.

Authors:  R Abonour; D A Williams; L Einhorn; K M Hall; J Chen; J Coffman; C M Traycoff; A Bank; I Kato; M Ward; S D Williams; R Hromas; M J Robertson; F O Smith; D Woo; B Mills; E F Srour; K Cornetta
Journal:  Nat Med       Date:  2000-06       Impact factor: 53.440

4.  Dose finding with retroviral vectors: correlation of retroviral vector copy numbers in single cells with gene transfer efficiency in a cell population.

Authors:  Olga S Kustikova; Anke Wahlers; Klaus Kuhlcke; Birgit Stahle; Axel R Zander; Christopher Baum; Boris Fehse
Journal:  Blood       Date:  2003-07-24       Impact factor: 22.113

5.  Quantitation of MLV-based retroviral vectors using real-time RT-PCR.

Authors:  M Carmo; C Peixoto; A S Coroadinha; P M Alves; P E Cruz; M J T Carrondo
Journal:  J Virol Methods       Date:  2004-08       Impact factor: 2.014

6.  CD34+ cell selection from frozen cord blood products using the Isolex 300i and CliniMACS CD34 selection devices.

Authors:  I K McNiece; G B Stoney; B P Kern; R A Briddell
Journal:  J Hematother       Date:  1998-10

7.  Comparison of retroviral transduction efficiency in CD34+ cells derived from bone marrow versus G-CSF-mobilized or G-CSF plus stem cell factor-mobilized peripheral blood in nonhuman primates.

Authors:  Peiman Hematti; Sascha Tuchman; Andre Larochelle; Mark E Metzger; Robert E Donahue; John F Tisdale
Journal:  Stem Cells       Date:  2004       Impact factor: 6.277

8.  Ex vivo expansion of frozen/thawed CD34+ cells isolated from frozen human apheresis products.

Authors:  J A Martinson; M Loudovaris; S L Smith; J G Bender; M Vachula; D E van Epps; H Kaizer; R G Ghalie; B C McLeod
Journal:  J Hematother       Date:  1997-02

9.  Comparative effects of suppressive cytokines on isolated single CD34(3+) stem/progenitor cells from human bone marrow and umbilical cord blood plated with and without serum.

Authors:  L Lu; M Xiao; S Grigsby; W X Wang; B Wu; R N Shen; H E Broxmeyer
Journal:  Exp Hematol       Date:  1993-10       Impact factor: 3.084

10.  Integration of murine leukemia virus DNA depends on mitosis.

Authors:  T Roe; T C Reynolds; G Yu; P O Brown
Journal:  EMBO J       Date:  1993-05       Impact factor: 11.598
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