OBJECTIVE: To investigate the allergic reaction in neonatal streptozotocin (nSTZ)-induced diabetes mellitus. MATERIAL: Male newborn Wistar rats were made diabetic by the injection of streptozotocin (160 mg/kg, i. p.) and used 8 weeks thereafter. TREATMENT: Animals were sensitized against ovalbumin (OA, 50 microg and Al(OH)3, 5 mg, s. c.) and challenged 14 or 21 days thereafter. METHODS: OA-induced airway inflammation and OA-induced pleurisy models were used to investigate leukocyte migration (total and differential leukocyte counts) and lung vascular permeability (Evans blue dye extravasation). RESULTS: nSTZ-diabetic rats presented glucose intolerance and insulin resistance. Relative to controls, nSTZ rats exhibited a 30% to 50% reduction in lung vascular permeability. Leukocyte infiltration in both models of allergen-induced inflammation, and number of pleural mast cells did not differ between groups. CONCLUSIONS: Data suggest that the reduction of allergic inflammatory reactions in nSTZ rats is restricted to microvascular dysfunctions and associated, probably, with insulin resistance in lung microvascular endothelium.
OBJECTIVE: To investigate the allergic reaction in neonatal streptozotocin (nSTZ)-induced diabetes mellitus. MATERIAL: Male newborn Wistar rats were made diabetic by the injection of streptozotocin (160 mg/kg, i. p.) and used 8 weeks thereafter. TREATMENT: Animals were sensitized against ovalbumin (OA, 50 microg and Al(OH)3, 5 mg, s. c.) and challenged 14 or 21 days thereafter. METHODS: OA-induced airway inflammation and OA-induced pleurisy models were used to investigate leukocyte migration (total and differential leukocyte counts) and lung vascular permeability (Evans blue dye extravasation). RESULTS:nSTZ-diabeticrats presented glucose intolerance and insulin resistance. Relative to controls, nSTZrats exhibited a 30% to 50% reduction in lung vascular permeability. Leukocyte infiltration in both models of allergen-induced inflammation, and number of pleural mast cells did not differ between groups. CONCLUSIONS: Data suggest that the reduction of allergic inflammatory reactions in nSTZrats is restricted to microvascular dysfunctions and associated, probably, with insulin resistance in lung microvascular endothelium.
Authors: Joilson O Martins; Carlos A L Campos; José W M C Cruz; Simone Manzolli; Venâncio A F Alves; Elcio O Vianna; Sonia Jancar; Paulina Sannomiya Journal: BMC Pulm Med Date: 2010-07-28 Impact factor: 3.317
Authors: Chad Jansen; Mark Speck; William E Greineisen; Kristina Maaetoft-Udsen; Edward Cordasco; Lori Mn Shimoda; Alexander J Stokes; Helen Turner Journal: J Immunobiol Date: 2017-12-11