OBJECTIVE: To examine the effect of bone morphogenetic protein 7 (BMP-7) on FSH receptor (FSHR) expression in human granulosa cells. DESIGN: Laboratory study using human samples. SETTING: University hospital. PATIENT(S): Human granulosa cells were obtained from 60 women undergoing oocyte retrieval for IVF. INTERVENTION(S): Human granulosa cells (GCs) were cultured with recombinant BMP-7, followed by RNA extraction. MAIN OUTCOME MEASURE(S): mRNA levels of GCs were measured by real-time reverse-transcription polymerase chain reaction. RESULT(S): Bone morphogenetic protein 7 increased FSHR gene expression in human luteinized granulosa cells, whereas it decreased LH receptor gene expression. Bone morphogenetic protein 7 also increased FSH-induced cyclic adenosine monophosphate production in GCs, indicating up-regulation of the cellular response to FSH. Although BMP-7 increased gene expression of activin-betaA and -betaB in GCs, inhibition of activin function did not affect the BMP-7-induced FSHR gene expression. CONCLUSION(S): These findings provide new insight into the biologic function of BMP-7 in the human ovary and demonstrate its unique mechanism of regulating FSHR action. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
OBJECTIVE: To examine the effect of bone morphogenetic protein 7 (BMP-7) on FSH receptor (FSHR) expression in human granulosa cells. DESIGN: Laboratory study using human samples. SETTING: University hospital. PATIENT(S): Human granulosa cells were obtained from 60 women undergoing oocyte retrieval for IVF. INTERVENTION(S): Human granulosa cells (GCs) were cultured with recombinant BMP-7, followed by RNA extraction. MAIN OUTCOME MEASURE(S): mRNA levels of GCs were measured by real-time reverse-transcription polymerase chain reaction. RESULT(S): Bone morphogenetic protein 7 increased FSHR gene expression in human luteinized granulosa cells, whereas it decreased LH receptor gene expression. Bone morphogenetic protein 7 also increased FSH-induced cyclic adenosine monophosphate production in GCs, indicating up-regulation of the cellular response to FSH. Although BMP-7 increased gene expression of activin-betaA and -betaB in GCs, inhibition of activin function did not affect the BMP-7-induced FSHR gene expression. CONCLUSION(S): These findings provide new insight into the biologic function of BMP-7 in the human ovary and demonstrate its unique mechanism of regulating FSHR action. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Mayara Morena Del Cambre Amaral Weller; Marina Rufino S Fortes; Laercio R Porto-Neto; Matthew Kelly; Bronwyn Venus; Lisa Kidd; João Paulo Arcelino do Rego; Sophia Edwards; Gry B Boe-Hansen; Emily Piper; Sigrid A Lehnert; Simone Eliza Facioni Guimarães; Stephen Stewart Moore Journal: Front Vet Sci Date: 2016-10-18
Authors: Sheena L P Regan; Phil G Knight; John L Yovich; Yee Leung; Frank Arfuso; Arun Dharmarajan Journal: Front Endocrinol (Lausanne) Date: 2018-03-02 Impact factor: 5.555