Literature DB >> 1910780

A simplified method for the production of recombinant baculovirus.

B B Goswami1, R I Glazer.   

Abstract

A simplified method for producing recombinant baculovirus for expression of foreign genes is described. The method utilizes insect cells infected with the wild-type virus before transfection with the plasmid transfer vector, instead of the standard procedure utilizing cotransfection with a plasmid and viral DNA. Recombinant virus is preselected by a limiting dilution dot-blot hybridization procedure, rather than by morphologic criteria alone. In addition, we have found that plasmid purification by anion-exchange chromatography is as efficacious for transfection as plasmid purified by cesium chloride density gradient centrifugation. These modifications allows for an efficient, rapid, inexpensive and more objective protocol for the selection of recombinant baculovirus compared to the conventional protocol.

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Year:  1991        PMID： 1910780

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  8 in total

1.  Rat urate oxidase produced by recombinant baculovirus expression: formation of peroxisome crystalloid core-like structures.

Authors:  K Alvares; R J Widrow; G M Abu-Jawdeh; J V Schmidt; A V Yeldandi; M S Rao; J K Reddy
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

2.  Transfection techniques for producing recombinant baculoviruses.

Authors:  K M Trotter; H A Wood
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

3.  Expression of feline immunodeficiency virus gag and env precursor proteins in Spodoptera frugiperda cells and their use in immunodiagnosis.

Authors:  E J Verschoor; A L van Vliet; H F Egberink; W Hesselink; M C Horzinek; A de Ronde
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

4.  Detection of hepatitis A virus in Mercenaria mercenaria by coupled reverse transcription and polymerase chain reaction.

Authors:  B B Goswami; W H Koch; T A Cebula
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

5.  Overexpression, purification, and characterization of full-length and mutant caldesmons using a baculovirus expression system.

Authors:  Z Wang; K Y Horiuchi; S S Jacob; S Gopalakurup; S Chacko
Journal:  J Muscle Res Cell Motil       Date:  1994-12       Impact factor: 2.698

6.  Activation of purified human protein kinase C alpha and beta I isoenzymes in vitro by Ca2+, phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate.

Authors:  G Kochs; R Hummel; B Fiebich; T F Sarre; D Marmé; H Hug
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

7.  Sheeppox virus kelch-like gene SPPV-019 affects virus virulence.

Authors:  C A Balinsky; G Delhon; C L Afonso; G R Risatti; M V Borca; R A French; E R Tulman; S J Geary; D L Rock
Journal:  J Virol       Date:  2007-08-08       Impact factor: 5.103

8.  Decrease in equilibrative uridine transport during monocytic differentiation of HL-60 leukaemia: involvement of protein kinase C.

Authors:  C W Lee
Journal:  Biochem J       Date:  1994-06-01       Impact factor: 3.857
  8 in total

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