Robert A Hirst1, Andrew Rutman, Christopher O'Callaghan. 1. Department of Infection, Immunity and Inflammation, University of Leicester, Robert Kilpatrick Clinical Sciences Building, Leicester Royal Infirmary, P.O. Box 65, Leicester LE2 7LX, UK.
Abstract
OBJECTIVES: Hydrogen peroxide [H(2)O(2): 3% w/v (1.1 M)] has been used as a haemostatic agent during neurosurgery applied to both the external and ventricular surface of the brain. We hypothesised that H(2)O(2) would be toxic to the ciliated ependyma, a single layer of cells that separates cerebrospinal fluid from the neuronal tissue of the brain. MATERIALS AND METHODS: The effect of H(2)O(2) was assessed by determining ependymal ciliary beat frequency (CBF) using high-speed video analysis and ultrastructure by electron microscopy. RESULTS: Brief exposure to H(2)O(2) caused cessation of ciliary beat frequency and extensive damage of the ependyma. CONCLUSIONS: Damage to the ciliated ependyma is of concern, as regeneration following damage is very poor and if breached underlying neuronal tissue and a population of neuronal progenitor cells that lie immediately beneath may also be exposed to H(2)O(2).
OBJECTIVES:Hydrogen peroxide [H(2)O(2): 3% w/v (1.1 M)] has been used as a haemostatic agent during neurosurgery applied to both the external and ventricular surface of the brain. We hypothesised that H(2)O(2) would be toxic to the ciliated ependyma, a single layer of cells that separates cerebrospinal fluid from the neuronal tissue of the brain. MATERIALS AND METHODS: The effect of H(2)O(2) was assessed by determining ependymal ciliary beat frequency (CBF) using high-speed video analysis and ultrastructure by electron microscopy. RESULTS: Brief exposure to H(2)O(2) caused cessation of ciliary beat frequency and extensive damage of the ependyma. CONCLUSIONS: Damage to the ciliated ependyma is of concern, as regeneration following damage is very poor and if breached underlying neuronal tissue and a population of neuronal progenitor cells that lie immediately beneath may also be exposed to H(2)O(2).
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