Xiao-dong Chen1, Jian-hua Lin. 1. Shenzhen Pingle Hospital of Orthopedics and Traumatology, Shenzhen 518010, Guangdong, China. cxdf22@163.com
Abstract
OBJECTIVE: To observe the function of pilose antler polypeptides (PAP) on replicative senescence of rat chondrocyte subcultivated in vitro by means of PAP intervention and controlled experiment. METHODS: The 3rd generation chondrocytes were divided into blank group, and PAP groups with three different concentration of PAP which were passaged to the 4th generation. Meanwhile, the 2nd generation of chontrocytes were used as control group. The chondrocytes in different groups were detected with the method of histochemistry for S-A-beta-gal, flow cytometry for cell life cycle and proliferation index, alcian blue test for the content and structure of GAG of ECM, and RT-PCR for type II collagen and Aggrecan. Then PAP's function was observed regarding the appearance and functional status in the process of chondrocyte's senescence. RESULTS: PAP significantly inhibited chondrocyte's express of S-A-beta-gal (P<0.01), promoted chondrocyte's proliferation (P<0.01), reduced cell content on G1 phase, enhanced the content of GAG, type II collagen and Aggrecan of ECM (P<0.01). CONCLUSION: PAP can significantly resist rat chondrocyte senescence occurred in subcultivation.
OBJECTIVE: To observe the function of pilose antler polypeptides (PAP) on replicative senescence of rat chondrocyte subcultivated in vitro by means of PAP intervention and controlled experiment. METHODS: The 3rd generation chondrocytes were divided into blank group, and PAP groups with three different concentration of PAP which were passaged to the 4th generation. Meanwhile, the 2nd generation of chontrocytes were used as control group. The chondrocytes in different groups were detected with the method of histochemistry for S-A-beta-gal, flow cytometry for cell life cycle and proliferation index, alcian blue test for the content and structure of GAG of ECM, and RT-PCR for type II collagen and Aggrecan. Then PAP's function was observed regarding the appearance and functional status in the process of chondrocyte's senescence. RESULTS: PAP significantly inhibited chondrocyte's express of S-A-beta-gal (P<0.01), promoted chondrocyte's proliferation (P<0.01), reduced cell content on G1 phase, enhanced the content of GAG, type II collagen and Aggrecan of ECM (P<0.01). CONCLUSION: PAP can significantly resist rat chondrocyte senescence occurred in subcultivation.