| Literature DB >> 19099247 |
Mariana Brocardo1, Beric R Henderson.
Abstract
The adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers. APC is a multi-domain protein whose distribution at different subcellular locations correlates with unique cellular processes. Our laboratory has focused on the link between APC subcellular location and function, and has characterized pathways for the trafficking of APC both into and out of the nucleus. Antibody specificity is an important factor in the determination of APC localization, and in this chapter we outline a strategy for the unambiguous detection of APC using a combination of biochemical and cell biology approaches.Entities:
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Year: 2008 PMID： 19099247 DOI： 10.1007/978-1-59745-249-6_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745