BACKGROUND: A novel chromogenic medium for isolation and identification of Pseudomonas aeruginosa from sputa of cystic fibrosis (CF) patients was evaluated and compared with standard laboratory methods. METHODS: One hundred sputum samples from distinct CF patients were cultured onto blood agar (BA), Pseudomonas CN selective agar (CN) and a Pseudomonas chromogenic medium (PS-ID). All Gram-negative morphological variants from each medium were subjected to antimicrobial susceptibility testing, and identification using a combination of biochemical and molecular methods. RESULTS: P. aeruginosa was isolated from 62 samples after 72 h incubation. Blood agar recovered P. aeruginosa from 56 samples (90.3%) compared with 59 samples (95.2%) using either CN or PS-ID. The positive predictive value of PS-ID (98.3%) was significantly higher than growth on CN (88.5%) for identification of P. aeruginosa (P<0.05). CONCLUSIONS: PS-ID is a promising medium allowing for the isolation and simultaneous identification of P. aeruginosa from sputa of CF patients.
BACKGROUND: A novel chromogenic medium for isolation and identification of Pseudomonas aeruginosa from sputa of cystic fibrosis (CF) patients was evaluated and compared with standard laboratory methods. METHODS: One hundred sputum samples from distinct CFpatients were cultured onto blood agar (BA), Pseudomonas CN selective agar (CN) and a Pseudomonas chromogenic medium (PS-ID). All Gram-negative morphological variants from each medium were subjected to antimicrobial susceptibility testing, and identification using a combination of biochemical and molecular methods. RESULTS:P. aeruginosa was isolated from 62 samples after 72 h incubation. Blood agar recovered P. aeruginosa from 56 samples (90.3%) compared with 59 samples (95.2%) using either CN or PS-ID. The positive predictive value of PS-ID (98.3%) was significantly higher than growth on CN (88.5%) for identification of P. aeruginosa (P<0.05). CONCLUSIONS:PS-ID is a promising medium allowing for the isolation and simultaneous identification of P. aeruginosa from sputa of CFpatients.
Authors: Thang V Truong; Alexander Twist; Andrey Zaytsev; Emma C L Marrs; Audrey Perry; Graeme Turnbull; Sylvain Orenga; Stephen P Stanforth; John D Perry Journal: Microorganisms Date: 2022-05-10
Authors: Ryan Thompson; Dominic Stephenson; Hannah E Sykes; John D Perry; Stephen P Stanforth; John R Dean Journal: RSC Adv Date: 2020-03-12 Impact factor: 4.036
Authors: Marie Cellier; Arthur L James; Sylvain Orenga; John D Perry; Graeme Turnbull; Stephen P Stanforth Journal: PLoS One Date: 2016-07-08 Impact factor: 3.240