| Literature DB >> 19094963 |
Peishi Yan1, Atsushi Nagasawa, Hideki Uosaki, Akihiro Sugimoto, Kohei Yamamizu, Mizue Teranishi, Hiroyuki Matsuda, Satoshi Matsuoka, Tadashi Ikeda, Masashi Komeda, Ryuzo Sakata, Jun K Yamashita.
Abstract
Though cardiac progenitor cells should be a suitable material for cardiac regeneration, efficient ways to induce cardiac progenitors from embryonic stem (ES) cells have not been established. Extending our systematic cardiovascular differentiation method of ES cells, here we show efficient and specific expansion of cardiomyocytes and highly cardiogenic progenitors from ES cells. An immunosuppressant, cyclosporin-A (CSA), showed a novel effect specifically acting on mesoderm cells to drastically increase cardiac progenitors as well as cardiomyocytes by 10-20 times. Approximately 200 cardiomyocytes could be induced from one mouse ES cell using this method. Expanded progenitors successfully integrated into scar tissue of infracted heart as cardiomyocytes after cell transplantation to rat myocardial infarction model. CSA elicited specific induction of cardiac lineage from mesoderm in a novel mesoderm-specific, NFAT independent fashion. This simple but efficient differentiation technology would be extended to induce pluripotent stem (iPS) cells and broadly contribute to cardiac regeneration.Entities:
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Year: 2008 PMID： 19094963 DOI： 10.1016/j.bbrc.2008.12.019
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575