AIM: The aim of this study was to determine the prevalence of 13 bacterial species in the dorsum tongue biofilm and compare its microbiota to that of subgingival samples in healthy adult subjects using DNA Checkerboard hybridization. METHODS: A total of 40 samples, from subgingival and tongue biofilm, were enrolled into the study. All the samples were harvested and analyzed by DNA Checkerboard method for their content of 13 species of bacteria. Bacterial scores from each subject and location were averaged and the results of both sites were compared using the Wilcoxon signed ranks test. RESULTS: All the microorganisms evaluated were detected in both tongue and subgingival samples, without significant differences in their prevalence. CONCLUSIONS: The dorsum of the tongue was colonized by various microorganisms with similar prevalence as found in the subgingival biofilm of healthy individuals, suggesting that the tongue could be a potential microbial reservoir and may play a role in the recolonization of teeth surfaces.
AIM: The aim of this study was to determine the prevalence of 13 bacterial species in the dorsum tongue biofilm and compare its microbiota to that of subgingival samples in healthy adult subjects using DNA Checkerboard hybridization. METHODS: A total of 40 samples, from subgingival and tongue biofilm, were enrolled into the study. All the samples were harvested and analyzed by DNA Checkerboard method for their content of 13 species of bacteria. Bacterial scores from each subject and location were averaged and the results of both sites were compared using the Wilcoxon signed ranks test. RESULTS: All the microorganisms evaluated were detected in both tongue and subgingival samples, without significant differences in their prevalence. CONCLUSIONS: The dorsum of the tongue was colonized by various microorganisms with similar prevalence as found in the subgingival biofilm of healthy individuals, suggesting that the tongue could be a potential microbial reservoir and may play a role in the recolonization of teeth surfaces.
Authors: Adi Binder Gallimidi; Stuart Fischman; Brurya Revach; Raanan Bulvik; Alina Maliutina; Ariel M Rubinstein; Gabriel Nussbaum; Michael Elkin Journal: Oncotarget Date: 2015-09-08
Authors: André Göhler; Adrian Hetzer; Birte Holtfreter; Marie Henrike Geisel; Carsten Oliver Schmidt; Ivo Steinmetz; Thomas Kocher Journal: PLoS One Date: 2014-07-16 Impact factor: 3.240
Authors: Vaia Galimanas; Michael William Hall; Natasha Singh; Michael David Joseph Lynch; Michael Goldberg; Howard Tenenbaum; Dennis Gerard Cvitkovitch; Josh David Neufeld; Dilani Braziunas Senadheera Journal: Microbiome Date: 2014-08-26 Impact factor: 14.650