Literature DB >> 19090352

Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles.

M Hema1, Ch V Subba Reddy, H S Savithri, P Sreenivasulu.   

Abstract

Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.

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Year:  2008        PMID: 19090352

Source DB:  PubMed          Journal:  Indian J Exp Biol        ISSN: 0019-5189            Impact factor:   0.818


  3 in total

1.  Cotranslational coat protein-mediated inhibition of potyviral RNA translation.

Authors:  Jane Besong-Ndika; Konstantin I Ivanov; Anders Hafrèn; Thierry Michon; Kristiina Mäkinen
Journal:  J Virol       Date:  2015-01-28       Impact factor: 5.103

Review 2.  Sharka: the past, the present and the future.

Authors:  Jiri Sochor; Petr Babula; Vojtech Adam; Boris Krska; Rene Kizek
Journal:  Viruses       Date:  2012-11-07       Impact factor: 5.048

Review 3.  Construction and characterization of virus-like particles: a review.

Authors:  Andris Zeltins
Journal:  Mol Biotechnol       Date:  2013-01       Impact factor: 2.695

  3 in total

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