Literature DB >> 19089332

Novel techniques for real-time monitoring of cGMP in living cells.

Viacheslav O Nikolaev1, Martin J Lohse.   

Abstract

Recent developments of biophysical and electrophysiological techniques have enabled researchers to monitor levels of free intracellular cGMP in real-time and in intact living cells. These techniques are based on the use of cGMP sensors, which respond to cGMP with changes in transmembrane ion current or changes in fluorescence. Here, we describe the principles of these techniques, compare them in terms of sensitivity and discuss possible application for current cell biology and physiology.

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Year:  2009        PMID： 19089332     DOI： 10.1007/978-3-540-68964-5_11

Source DB:  PubMed          Journal:  Handb Exp Pharmacol        ISSN： 0171-2004


  9 in total

1.  FRET microscopy for real-time monitoring of signaling events in live cells using unimolecular biosensors.

Authors:  Julia U Sprenger; Ruwan K Perera; Konrad R Götz; Viacheslav O Nikolaev
Journal:  J Vis Exp       Date:  2012-08-20       Impact factor: 1.355

2.  Transgenic mice for cGMP imaging.

Authors:  Martin Thunemann; Lai Wen; Matthias Hillenbrand; Angelos Vachaviolos; Susanne Feil; Thomas Ott; Xiaoxing Han; Dai Fukumura; Rakesh K Jain; Michael Russwurm; Cor de Wit; Robert Feil
Journal:  Circ Res       Date:  2013-06-25       Impact factor: 17.367

3.  In vivo genetic dissection of O2-evoked cGMP dynamics in a Caenorhabditis elegans gas sensor.

Authors:  Africa Couto; Shigekazu Oda; Viacheslav O Nikolaev; Zoltan Soltesz; Mario de Bono
Journal:  Proc Natl Acad Sci U S A       Date:  2013-08-12       Impact factor: 11.205

4.  Fluorescence dequenching makes haem-free soluble guanylate cyclase detectable in living cells.

Authors:  Linda S Hoffmann; Peter M Schmidt; Yvonne Keim; Carsten Hoffmann; Harald H H W Schmidt; Johannes-Peter Stasch
Journal:  PLoS One       Date:  2011-08-17       Impact factor: 3.240

5.  Correlative intravital imaging of cGMP signals and vasodilation in mice.

Authors:  Martin Thunemann; Kjestine Schmidt; Cor de Wit; Xiaoxing Han; Rakesh K Jain; Dai Fukumura; Robert Feil
Journal:  Front Physiol       Date:  2014-10-14       Impact factor: 4.566

Review 6.  Imaging of PDE2- and PDE3-Mediated cGMP-to-cAMP Cross-Talk in Cardiomyocytes.

Authors:  Nikoleta Pavlaki; Viacheslav O Nikolaev
Journal:  J Cardiovasc Dev Dis       Date:  2018-01-19

Review 7.  cGMP Signaling and Vascular Smooth Muscle Cell Plasticity.

Authors:  Moritz Lehners; Hyazinth Dobrowinski; Susanne Feil; Robert Feil
Journal:  J Cardiovasc Dev Dis       Date:  2018-04-19

8.  CNP regulates cardiac contractility and increases cGMP near both SERCA and TnI: difference from BNP visualized by targeted cGMP biosensors.

Authors:  Ornella Manfra; Gaia Calamera; Alexander Froese; Dulasi Arunthavarajah; Nicoletta C Surdo; Silja Meier; Arne Olav Melleby; Monica Aasrum; Jan Magnus Aronsen; Viacheslav O Nikolaev; Manuela Zaccolo; Lise Román Moltzau; Finn Olav Levy; Kjetil Wessel Andressen
Journal:  Cardiovasc Res       Date:  2022-05-06       Impact factor: 13.081

Review 9.  Biophysical techniques for detection of cAMP and cGMP in living cells.

Authors:  Julia U Sprenger; Viacheslav O Nikolaev
Journal:  Int J Mol Sci       Date:  2013-04-12       Impact factor: 5.923
  9 in total

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