Microheterogeneity of the alpha subunits of G proteins in bovine brain clathrin coated vesicles.

Clathrin coated vesicles (CV) from bovine brain express the various alpha subunits of guanine nucleotide regulatory proteins (G-proteins): Go, Gi 1, 2, 3 and Gs. Western blot analysis using two different antisera revealed the presence of the 39-kDa Go alpha subunit in CV. Antisera RM/1, in turn, demonstrated the expression of the short (45 kDa) and long (52 kDa) form variants of the Gs alpha subunit. The analysis demonstrates that the heterogeneity of alpha subunits of G-proteins in bovine brain tissue is also reflected in the CV transport organelles.