CONTEXT: Although diurnal variation of testosterone and other hormones in men has been well documented, the effect of this variation on sampling during typical clinic hours has not been examined. OBJECTIVE: Our objective was to examine temporal variation in serum testosterone and five other hormones in men over normal clinic hours. DESIGN: Blood samples were collected at six separate visits, three morning visits 1-3 d apart and three afternoon visits 1-3 d apart. SETTING AND PARTICIPANTS: In Boston, MA, 66 men participated, 30-80 yr of age, randomly selected from the Boston Area Community Health Survey who completed at least five visits. MAIN OUTCOME MEASURES: The age-specific ratio of hormone level at times ranging from 0801-1600 h to hormone level at 0800 h was calculated. Ratios were calculated from parameter estimates obtained from cosinor models. RESULTS: In men 30-40 yr old, testosterone levels were 20-25% lower at 1600 h than at 0800 h. The difference declined with age, with a 10% difference at 70 yr. 17 men with at least one of three measurements less than 300 ng/dl (10.4 nmol/liter) after 1200 h had normal testosterone levels at all three visits before 1200 h (five of eight men 30-47 yr old, four of nine men 66-80 yr old). Much lower levels of diurnal variation were found for dihydrotestosterone, SHBG, LH, FSH, and estradiol at all ages. CONCLUSIONS: Our results support the recommendation of restricting testosterone measurements to morning hours in both young and older men. Limited diurnal variation in other hormones indicates that sampling through the day is appropriate.
CONTEXT: Although diurnal variation of testosterone and other hormones in men has been well documented, the effect of this variation on sampling during typical clinic hours has not been examined. OBJECTIVE: Our objective was to examine temporal variation in serum testosterone and five other hormones in men over normal clinic hours. DESIGN: Blood samples were collected at six separate visits, three morning visits 1-3 d apart and three afternoon visits 1-3 d apart. SETTING AND PARTICIPANTS: In Boston, MA, 66 men participated, 30-80 yr of age, randomly selected from the Boston Area Community Health Survey who completed at least five visits. MAIN OUTCOME MEASURES: The age-specific ratio of hormone level at times ranging from 0801-1600 h to hormone level at 0800 h was calculated. Ratios were calculated from parameter estimates obtained from cosinor models. RESULTS: In men 30-40 yr old, testosterone levels were 20-25% lower at 1600 h than at 0800 h. The difference declined with age, with a 10% difference at 70 yr. 17 men with at least one of three measurements less than 300 ng/dl (10.4 nmol/liter) after 1200 h had normal testosterone levels at all three visits before 1200 h (five of eight men 30-47 yr old, four of nine men 66-80 yr old). Much lower levels of diurnal variation were found for dihydrotestosterone, SHBG, LH, FSH, and estradiol at all ages. CONCLUSIONS: Our results support the recommendation of restricting testosterone measurements to morning hours in both young and older men. Limited diurnal variation in other hormones indicates that sampling through the day is appropriate.
Authors: Michael J Diver; Komal E Imtiaz; Aftab M Ahmad; Jiten P Vora; William D Fraser Journal: Clin Endocrinol (Oxf) Date: 2003-06 Impact factor: 3.478
Authors: F A Lévi; C Canon; Y Touitou; J Sulon; M Mechkouri; E D Ponsart; J P Touboul; J M Vannetzel; I Mowzowicz; A Reinberg Journal: Clin Exp Immunol Date: 1988-02 Impact factor: 4.330
Authors: G Y Nicolau; E Haus; D J Lakatua; C Bogdan; L Sackett-Lundeen; M Popescu; H Berg; E Petrescu; E Robu Journal: Endocrinologie Date: 1985 Oct-Dec
Authors: Manthos G Giannoulis; Finbarr C Martin; K Sreekumaran Nair; A Margot Umpleby; Peter Sonksen Journal: Endocr Rev Date: 2012-03-20 Impact factor: 19.871
Authors: Tuong-Vi Nguyen; James T McCracken; Matthew D Albaugh; Kelly N Botteron; James J Hudziak; Simon Ducharme Journal: Psychoneuroendocrinology Date: 2015-09-25 Impact factor: 4.905
Authors: Brian R Stolze; Verena Gounden; Jianghong Gu; Elizabeth A Elliott; Likhona S Masika; Brent S Abel; Deborah P Merke; Monica C Skarulis; Steven J Soldin Journal: J Steroid Biochem Mol Biol Date: 2015-12-22 Impact factor: 4.292
Authors: Richard V Clark; Ann C Walker; Susan Andrews; Philip Turnbull; Jeffrey A Wald; Mindy H Magee Journal: Br J Clin Pharmacol Date: 2017-06-10 Impact factor: 4.335
Authors: Katherine E Joyce; Mary L Biggs; Luc Djoussé; Joachim H Ix; Jorge R Kizer; David S Siscovick; Molly M Shores; Alvin M Matsumoto; Kenneth J Mukamal Journal: J Clin Endocrinol Metab Date: 2017-01-01 Impact factor: 5.958
Authors: Tuong-Vi Nguyen; James T McCracken; Simon Ducharme; Brett F Cropp; Kelly N Botteron; Alan C Evans; Sherif Karama Journal: J Neurosci Date: 2013-06-26 Impact factor: 6.167
Authors: Tuong-Vi Nguyen; Jimin Lew; Matthew D Albaugh; Kelly N Botteron; James J Hudziak; Vladimir S Fonov; D Louis Collins; Simon Ducharme; James T McCracken Journal: Psychoneuroendocrinology Date: 2016-12-08 Impact factor: 4.905
Authors: Brian H Chen; Kathleen Brennan; Atsushi Goto; Yiqing Song; Najib Aziz; Nai-chieh Y You; Melissa F Wellons; JoAnn E Manson; Donna L White; Anthony W Butch; Simin Liu Journal: Clin Chem Date: 2012-08-20 Impact factor: 8.327