BACKGROUND: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. METHOD: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. RESULTS: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. CONCLUSION: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.
BACKGROUND: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. METHOD: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. RESULTS: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. CONCLUSION: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.
Authors: Alexey N Semenov; Boris P Yakimov; Anna A Rubekina; Dmitry A Gorin; Vladimir P Drachev; Mikhail P Zarubin; Alexander N Velikanov; Juergen Lademann; Victor V Fadeev; Alexander V Priezzhev; Maxim E Darvin; Evgeny A Shirshin Journal: Molecules Date: 2020-04-17 Impact factor: 4.411
Authors: Violina Baranauskaite Barbosa; Célia F Rodrigues; Laura Cerqueira; João M Miranda; Nuno F Azevedo Journal: Front Bioeng Biotechnol Date: 2022-09-23