Gokilaht-S 5EC testing on Culex quinquefasciatus Say larvae for an early detection in esterase and monooxygenase resistance system.

In this paper, we have studied the efficacy of Gokilaht-S 5EC (d,d-trans-cyphenothrin), a synthetic pyrethroid, when applied on all larval instars of Culex quinquefasciatus Say in laboratory conditions. We could observe this with six statistically significant concentrations at 0.0005, 0.0025, 0.075, 0.075, 0.1, and 0.2 microL/L, respectively. The esterase was detected by SDS-PAGE analysis and monooxygenase with microtiter plate assay. The esterase and monooxygenase were detected at concentrations of 0.005 and 0.075 microL/L, respectively. The outcome of these assays was discussed and we could see a pattern for early detection spectra to be proposed with these findings.