Literature DB >> 1908396

Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.

A Kuroda1, M Imazeki, J Sekiguchi.   

Abstract

A cell wall hydrolase of Bacillus subtilis was prepared from Escherichia coli cells harboring a plasmid containing the B. subtilis cwlA gene and purified by hydroxyapatite column chromatography and HPLC through TSK-gel G3000SWXL. In contrast to the molecular mass of 29,919 Da deduced from its nucleotide sequence, the purified CWLA is a 23 kDa protein. Characterization of the specific substrate bond cleaved by CWLA indicated the enzyme is an N-acetylmuramyl-L-alanine amidase. A 32-kDa precursor protein was detected on zymography of a crude cell homogenate. Some of the enzymatic properties of CWLA are also described.

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Year:  1991        PMID： 1908396     DOI： 10.1016/0378-1097(91)90462-j

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  8 in total

1.  Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.

Authors:  R Ohnishi; S Ishikawa; J Sekiguchi
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

2.  Identification of the structural genes for N-acetylmuramoyl-L-alanine amidase and its modifier in Bacillus subtilis 168: inactivation of these genes by insertional mutagenesis has no effect on growth or cell separation.

Authors:  P Margot; D Karamata
Journal:  Mol Gen Genet       Date:  1992-04

3.  Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.

Authors:  A Kuroda; J Sekiguchi
Journal:  J Bacteriol       Date:  1991-11       Impact factor: 3.490

4.  Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.

Authors:  A Kuroda; Y Asami; J Sekiguchi
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

5.  High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation.

Authors:  A Kuroda; J Sekiguchi
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

6.  Characterization of the involvement of two compensatory autolysins in mother cell lysis during sporulation of Bacillus subtilis 168.

Authors:  T J Smith; S J Foster
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

7.  Genetic structure, isolation and characterization of a Bacillus licheniformis cell wall hydrolase.

Authors:  A Kuroda; Y Sugimoto; T Funahashi; J Sekiguchi
Journal:  Mol Gen Genet       Date:  1992-07

8.  Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis.

Authors:  Y Oda; R Nakayama; A Kuroda; J Sekiguchi
Journal:  Mol Gen Genet       Date:  1993-11
  8 in total

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