Literature DB >> 19083758

Q8--a short amplicon multiplex including the German DNA database systems.

Kathrin Müller1, Rachel Klein, Erich Miltner, Peter Wiegand.   

Abstract

We have developed a modified STR (short tandem repeat) concept including the eight German DNA database systems D3S1358, FGA, TH01, VWA, SE33, D8S1179, D18S51, D21S11 and the gender typing system Amelogenin (Q8). The Q8 is well suitable especially for weak and degraded stains. Compared to commercially established DNA database kits the amplicon lengths were significantly reduced. To optimise the typing success for weak stains the most sensitive fluorescent dyes FAM, JOE and TET were used. By comparing the Q8 kit with the MPX2 kit, a well-established commercial kit, a higher success rate was reached using our concept.

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Year:  2007        PMID: 19083758     DOI: 10.1016/j.fsigen.2007.02.005

Source DB:  PubMed          Journal:  Forensic Sci Int Genet        ISSN: 1872-4973            Impact factor:   4.882


  3 in total

1.  Development and validation of I-DNA1: a 15-Loci multiplex system for identity testing.

Authors:  A Odriozola; J M Aznar; D Celorrio; M L Bravo; J J Builes; J F Val-Bernal; Marian M de Pancorbo
Journal:  Int J Legal Med       Date:  2011-01-22       Impact factor: 2.686

2.  Hot flakes in cold cases.

Authors:  Harald Schneider; Thomas Sommerer; Steve Rand; Peter Wiegand
Journal:  Int J Legal Med       Date:  2011-01-22       Impact factor: 2.686

3.  As solid as a rock-comparison of CE- and MPS-based analyses of the petrosal bone as a source of DNA for forensic identification of challenging cranial bones.

Authors:  Galina Kulstein; Thorsten Hadrys; Peter Wiegand
Journal:  Int J Legal Med       Date:  2017-07-28       Impact factor: 2.686

  3 in total

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