Literature DB >> 19083728

MiniY-STR quadruplex systems with short amplicon lengths for analysis of degraded DNA samples.

H Asamura1, H Sakai, M Ota, H Fukushima.   

Abstract

Two short amplicon Y-chromosomal short tandem repeat (miniY-STR) quadruplex systems for the eight Y-STR loci DYS522, DYS508, DYS632, DYS556, DYS570, DYS576, DYS504 and DYS540 were devised using newly designed primer sets. Among 224 samples from Japanese population, amplification product lengths detected in these Y-STR loci ranged from 95 to 147bp, while 170 different haplotype were identified (discrimination capacity=0.7589 and haplotype diversity=0.9949). As a result of test on degraded DNA samples using the miniY-STR quadruplex systems, the systems proved to be an quite effective tools for analyzing degraded DNAs. We conclude that analyses of the miniY-STR quadruplex systems in addition to the commercial available Y-STR multiplex kits are highly useful for forensic practices of degraded DNA samples.

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Year:  2006        PMID: 19083728     DOI: 10.1016/j.fsigen.2006.10.008

Source DB:  PubMed          Journal:  Forensic Sci Int Genet        ISSN: 1872-4973            Impact factor:   4.882


  2 in total

1.  Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci.

Authors:  H Asamura; S Fujimori; M Ota; T Oki; H Fukushima
Journal:  Int J Legal Med       Date:  2007-09-26       Impact factor: 2.686

2.  Haplotype diversity of 23 Y-chromosomal STRs in a population sample from the Federal District (Brazil)--a territory that arose from nothing.

Authors:  Gustavo Barcelos Barra; Camilla Figueiredo Chianca; Ticiane Henriques Santa Rita; Lara Franciele Ribeiro Velasco; Claudia Ferreira de Souza
Journal:  Int J Legal Med       Date:  2014-01-05       Impact factor: 2.686

  2 in total

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