Literature DB >> 19082549

Determination of bacteriophage genome size by pulsed-field gel electrophoresis.

Erika Lingohr1, Shelley Frost, Roger P Johnson.   

Abstract

Standard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40-50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.

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Year:  2009        PMID： 19082549     DOI： 10.1007/978-1-60327-565-1_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Genome survey sequencing and genetic diversity of cultivated Akebia trifoliata assessed via phenotypes and SSR markers.

Authors:  Zheng Zhang; Jiawen Zhang; Qing Yang; Bin Li; Wen Zhou; Zhezhi Wang
Journal:  Mol Biol Rep       Date:  2021-01-05       Impact factor: 2.316

2.  Detection and differential identification of typhoidal Salmonella using bacteriophages and resazurin.

Authors:  Aniruddha Vaidya; Shashidhar Ravindranath; Uday S Annapure
Journal:  3 Biotech       Date:  2020-04-09       Impact factor: 2.406

3.  Isolation and characterisation of lytic bacteriophages of Klebsiella pneumoniae and Klebsiella oxytoca.

Authors:  Natia Karumidze; Ia Kusradze; Sophio Rigvava; Marine Goderdzishvili; Kumar Rajakumar; Zemphira Alavidze
Journal:  Curr Microbiol       Date:  2012-11-11       Impact factor: 2.188

4.  Global transcriptional response of Clostridium difficile carrying the CD38 prophage.

Authors:  Ognjen Sekulovic; Louis-Charles Fortier
Journal:  Appl Environ Microbiol       Date:  2015-02       Impact factor: 4.792

5.  Silage collected from dairy farms harbors an abundance of listeriaphages with considerable host range and genome size diversity.

Authors:  Kitiya Vongkamjan; Andrea Moreno Switt; Henk C den Bakker; Esther D Fortes; Martin Wiedmann
Journal:  Appl Environ Microbiol       Date:  2012-10-05       Impact factor: 4.792

6.  Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei.

Authors:  Jiraporn Gatedee; Kanyanan Kritsiriwuthinan; Edouard E Galyov; Jinyu Shan; Elena Dubinina; Narin Intarak; Martha R J Clokie; Sunee Korbsrisate
Journal:  Virol J       Date:  2011-07-26       Impact factor: 4.099

7.  Genomic, proteomic and physiological characterization of a T5-like bacteriophage for control of Shiga toxin-producing Escherichia coli O157:H7.

Authors:  Yan D Niu; Kim Stanford; Andrew M Kropinski; Hans-Wolfgang Ackermann; Roger P Johnson; Yi-Min She; Rafiq Ahmed; Andre Villegas; Tim A McAllister
Journal:  PLoS One       Date:  2012-04-13       Impact factor: 3.240

8.  The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5.

Authors:  Andrew M Kropinski; Tom Waddell; Juncai Meng; Kristyn Franklin; Hans-Wolfgang Ackermann; Rafiq Ahmed; Amanda Mazzocco; John Yates; Erika J Lingohr; Roger P Johnson
Journal:  Virol J       Date:  2013-03-06       Impact factor: 4.099

9.  Genome sequence and analysis of a broad-host range lytic bacteriophage that infects the Bacillus cereus group.

Authors:  Tarek F El-Arabi; Mansel W Griffiths; Yi-Min She; Andre Villegas; Erika J Lingohr; Andrew M Kropinski
Journal:  Virol J       Date:  2013-02-07       Impact factor: 4.099

10.  Endemic bacteriophages: a cautionary tale for evaluation of bacteriophage therapy and other interventions for infection control in animals.

Authors:  Andrew M Kropinski; Erika J Lingohr; Dianne M Moyles; Shivani Ojha; Amanda Mazzocco; Yi-Min She; Susan J Bach; Erica A Rozema; Kim Stanford; Tim A McAllister; Roger P Johnson
Journal:  Virol J       Date:  2012-09-17       Impact factor: 4.099
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